Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24951 | 3' | -62.7 | NC_005284.1 | + | 4553 | 0.69 | 0.196241 |
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Target: 5'- uACCGCCGCCG-CGAGCcagcuUuuGCUCg -3' miRNA: 3'- uUGGCGGUGGCgGCUCGc----AggCGAGa -5' |
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| 24951 | 3' | -62.7 | NC_005284.1 | + | 21749 | 0.71 | 0.159366 |
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Target: 5'- cGGCgGCCACCGCCGAcGCGgCgGCaUCg -3' miRNA: 3'- -UUGgCGGUGGCGGCU-CGCaGgCG-AGa -5' |
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| 24951 | 3' | -62.7 | NC_005284.1 | + | 6031 | 0.71 | 0.151178 |
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Target: 5'- gGAUCGgCGCCaGCCGuGGCGUCCGCa-- -3' miRNA: 3'- -UUGGCgGUGG-CGGC-UCGCAGGCGaga -5' |
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| 24951 | 3' | -62.7 | NC_005284.1 | + | 10160 | 0.72 | 0.122124 |
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Target: 5'- -cCCGCCGCggucgcgguCGCCGcGCGuUCCGCUCg -3' miRNA: 3'- uuGGCGGUG---------GCGGCuCGC-AGGCGAGa -5' |
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| 24951 | 3' | -62.7 | NC_005284.1 | + | 46446 | 0.78 | 0.041589 |
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Target: 5'- cGAUCGCCgucgccgaauGCCGCCGAGCGUCCGacgCUg -3' miRNA: 3'- -UUGGCGG----------UGGCGGCUCGCAGGCga-GA- -5' |
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| 24951 | 3' | -62.7 | NC_005284.1 | + | 18268 | 0.83 | 0.019926 |
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Target: 5'- cAGCCGCCGCCGUCGAaCGUCCGcCUCUc -3' miRNA: 3'- -UUGGCGGUGGCGGCUcGCAGGC-GAGA- -5' |
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| 24951 | 3' | -62.7 | NC_005284.1 | + | 36570 | 1.04 | 0.000416 |
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Target: 5'- cAACCGCCACCGCCGAGCGUCCGCUCUu -3' miRNA: 3'- -UUGGCGGUGGCGGCUCGCAGGCGAGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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