Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
24952 | 5' | -54.5 | NC_005284.1 | + | 50940 | 0.67 | 0.699688 |
Target: 5'- uGCGGG-GGAUG-UCGUGAgcgCUCAUCu -3' miRNA: 3'- gCGUCCaUCUGCgGGCACUa--GAGUAG- -5' |
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24952 | 5' | -54.5 | NC_005284.1 | + | 48010 | 0.71 | 0.482365 |
Target: 5'- uCGCGGGcguGACGCgCGUGAccgUgUCGUCg -3' miRNA: 3'- -GCGUCCau-CUGCGgGCACU---AgAGUAG- -5' |
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24952 | 5' | -54.5 | NC_005284.1 | + | 16297 | 0.71 | 0.462044 |
Target: 5'- gCGCAGGgcGA-GCCgGUGccgCUCGUCa -3' miRNA: 3'- -GCGUCCauCUgCGGgCACua-GAGUAG- -5' |
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24952 | 5' | -54.5 | NC_005284.1 | + | 9509 | 0.76 | 0.24667 |
Target: 5'- gCGUGGGUcGGCGCCCGgcGAgcgCUCGUCg -3' miRNA: 3'- -GCGUCCAuCUGCGGGCa-CUa--GAGUAG- -5' |
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24952 | 5' | -54.5 | NC_005284.1 | + | 37126 | 1.11 | 0.000924 |
Target: 5'- gCGCAGGUAGACGCCCGUGAUCUCAUCg -3' miRNA: 3'- -GCGUCCAUCUGCGGGCACUAGAGUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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