Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24958 | 5' | -56.6 | NC_005284.1 | + | 8330 | 0.66 | 0.672186 |
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Target: 5'- gCGUCgGACGCUcGGCGgcauucGGCgacgGCgaUCGg -3' miRNA: 3'- -GCAGgCUGCGA-CCGCaa----UCGa---CG--AGC- -5' |
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| 24958 | 5' | -56.6 | NC_005284.1 | + | 17759 | 0.66 | 0.650498 |
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Target: 5'- -cUCgGGCGCUGGCu---GCUGCUg- -3' miRNA: 3'- gcAGgCUGCGACCGcaauCGACGAgc -5' |
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| 24958 | 5' | -56.6 | NC_005284.1 | + | 14566 | 0.67 | 0.596157 |
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Target: 5'- -uUUCGGCGCUGuGCGUU-GC-GCUUGg -3' miRNA: 3'- gcAGGCUGCGAC-CGCAAuCGaCGAGC- -5' |
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| 24958 | 5' | -56.6 | NC_005284.1 | + | 33126 | 0.68 | 0.518401 |
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Target: 5'- cCG-CCGACGC-GGCGgcaucgUGcgcggcgaucgcacGCUGCUCGg -3' miRNA: 3'- -GCaGGCUGCGaCCGCa-----AU--------------CGACGAGC- -5' |
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| 24958 | 5' | -56.6 | NC_005284.1 | + | 36093 | 0.71 | 0.359718 |
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Target: 5'- uCGUCCGGUGCgGGCGcgAGCaGCUCc -3' miRNA: 3'- -GCAGGCUGCGaCCGCaaUCGaCGAGc -5' |
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| 24958 | 5' | -56.6 | NC_005284.1 | + | 33967 | 0.76 | 0.186683 |
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Target: 5'- uCGgCCGGCGCUGcuGCG--GGCUGCUCGa -3' miRNA: 3'- -GCaGGCUGCGAC--CGCaaUCGACGAGC- -5' |
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| 24958 | 5' | -56.6 | NC_005284.1 | + | 46472 | 1.08 | 0.000868 |
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Target: 5'- gCGUCCGACGCUGGCGUUAGCUGCUCGu -3' miRNA: 3'- -GCAGGCUGCGACCGCAAUCGACGAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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