Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24960 | 3' | -56.7 | NC_005284.1 | + | 49646 | 0.68 | 0.503554 |
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Target: 5'- gGCGUuccgaCGGUUCGAUCGAgcgagcGGUCGAg -3' miRNA: 3'- aCGCGg----GCCAGGCUAGCUa-----CUAGCUa -5' |
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| 24960 | 3' | -56.7 | NC_005284.1 | + | 50429 | 0.68 | 0.483121 |
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Target: 5'- cGCGCCaCGGgaaggCCGGggCGAUGAaggCGAg -3' miRNA: 3'- aCGCGG-GCCa----GGCUa-GCUACUa--GCUa -5' |
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| 24960 | 3' | -56.7 | NC_005284.1 | + | 30381 | 0.68 | 0.483121 |
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Target: 5'- aUGCGCUCGGcCCGcgUGugcUGGUCGGc -3' miRNA: 3'- -ACGCGGGCCaGGCuaGCu--ACUAGCUa -5' |
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| 24960 | 3' | -56.7 | NC_005284.1 | + | 32143 | 0.69 | 0.463095 |
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Target: 5'- cGUGUgCGG-CCGAUCGuaacGAUCGAUg -3' miRNA: 3'- aCGCGgGCCaGGCUAGCua--CUAGCUA- -5' |
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| 24960 | 3' | -56.7 | NC_005284.1 | + | 27494 | 0.69 | 0.443514 |
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Target: 5'- uUGCGCCCa---CGGUggCGAUGAUCGAUg -3' miRNA: 3'- -ACGCGGGccagGCUA--GCUACUAGCUA- -5' |
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| 24960 | 3' | -56.7 | NC_005284.1 | + | 37727 | 0.73 | 0.283661 |
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Target: 5'- aGCGCCgucaucggauagCGGagcuUCCGAUCGAUGAucUCGGUa -3' miRNA: 3'- aCGCGG------------GCC----AGGCUAGCUACU--AGCUA- -5' |
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| 24960 | 3' | -56.7 | NC_005284.1 | + | 7923 | 0.73 | 0.25642 |
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Target: 5'- gGCGCaaGGUCCGGUCGGuUGG-CGAUa -3' miRNA: 3'- aCGCGggCCAGGCUAGCU-ACUaGCUA- -5' |
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| 24960 | 3' | -56.7 | NC_005284.1 | + | 42008 | 0.75 | 0.208396 |
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Target: 5'- cGCGCCCGcaugCCGAUCGAgGAaaUCGAUg -3' miRNA: 3'- aCGCGGGCca--GGCUAGCUaCU--AGCUA- -5' |
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| 24960 | 3' | -56.7 | NC_005284.1 | + | 46827 | 1.06 | 0.001155 |
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Target: 5'- uUGCGCCCGGUCCGAUCGAUGAUCGAUg -3' miRNA: 3'- -ACGCGGGCCAGGCUAGCUACUAGCUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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