Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24960 | 5' | -55.3 | NC_005284.1 | + | 9638 | 0.66 | 0.670597 |
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Target: 5'- -cGUCGCCGugcgUCGACCacGCCUUGCc -3' miRNA: 3'- aaUAGCGGUu---GGCUGGccUGGAACGc -5' |
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| 24960 | 5' | -55.3 | NC_005284.1 | + | 37292 | 0.66 | 0.670597 |
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Target: 5'- aUAUCgGCCGACgCGACCaccGACCcgGCGc -3' miRNA: 3'- aAUAG-CGGUUG-GCUGGc--CUGGaaCGC- -5' |
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| 24960 | 5' | -55.3 | NC_005284.1 | + | 11309 | 0.66 | 0.681582 |
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Target: 5'- ---gCGCgacagUAGCCGGUCGGAUCUUGCGc -3' miRNA: 3'- aauaGCG-----GUUGGCUGGCCUGGAACGC- -5' |
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| 24960 | 5' | -55.3 | NC_005284.1 | + | 18769 | 0.66 | 0.681582 |
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Target: 5'- -aAUCGCCAguACCGcCCGuGCUcgUGCGa -3' miRNA: 3'- aaUAGCGGU--UGGCuGGCcUGGa-ACGC- -5' |
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| 24960 | 5' | -55.3 | NC_005284.1 | + | 787 | 0.66 | 0.703398 |
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Target: 5'- uUUAUCGagcaggaCGGCC-ACCGGGCCUuUGCc -3' miRNA: 3'- -AAUAGCg------GUUGGcUGGCCUGGA-ACGc -5' |
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| 24960 | 5' | -55.3 | NC_005284.1 | + | 20835 | 0.66 | 0.714206 |
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Target: 5'- ---gCGCCGGCCGACgagCGGGCg--GCGc -3' miRNA: 3'- aauaGCGGUUGGCUG---GCCUGgaaCGC- -5' |
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| 24960 | 5' | -55.3 | NC_005284.1 | + | 25072 | 0.66 | 0.724933 |
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Target: 5'- ---aCGCCugaugacGCCGGCCG-ACCUcGCGa -3' miRNA: 3'- aauaGCGGu------UGGCUGGCcUGGAaCGC- -5' |
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| 24960 | 5' | -55.3 | NC_005284.1 | + | 22071 | 0.71 | 0.407514 |
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Target: 5'- ----gGCUAugCGGCCGGACUgcGCGa -3' miRNA: 3'- aauagCGGUugGCUGGCCUGGaaCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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