Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24960 | 5' | -55.3 | NC_005284.1 | + | 36780 | 0.69 | 0.486171 |
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Target: 5'- gUGUCGCCGucgACCGACUuGACUgcGCGc -3' miRNA: 3'- aAUAGCGGU---UGGCUGGcCUGGaaCGC- -5' |
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| 24960 | 5' | -55.3 | NC_005284.1 | + | 40310 | 0.7 | 0.465797 |
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Target: 5'- ---cCGUagAGCCGACCGGuugcucCCUUGCGg -3' miRNA: 3'- aauaGCGg-UUGGCUGGCCu-----GGAACGC- -5' |
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| 24960 | 5' | -55.3 | NC_005284.1 | + | 8334 | 0.7 | 0.465797 |
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Target: 5'- -aAUCGCCGguugcagacgAUCGugCGGAUCUgcaUGCGg -3' miRNA: 3'- aaUAGCGGU----------UGGCugGCCUGGA---ACGC- -5' |
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| 24960 | 5' | -55.3 | NC_005284.1 | + | 42123 | 0.71 | 0.426439 |
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Target: 5'- gUGUaGCCGucGCCGaACUGGGCUUUGCGc -3' miRNA: 3'- aAUAgCGGU--UGGC-UGGCCUGGAACGC- -5' |
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| 24960 | 5' | -55.3 | NC_005284.1 | + | 22071 | 0.71 | 0.407514 |
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Target: 5'- ----gGCUAugCGGCCGGACUgcGCGa -3' miRNA: 3'- aauagCGGUugGCUGGCCUGGaaCGC- -5' |
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| 24960 | 5' | -55.3 | NC_005284.1 | + | 15286 | 0.73 | 0.329188 |
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Target: 5'- ----aGCCGACCGACgaGGACCgcacgaugUGCGg -3' miRNA: 3'- aauagCGGUUGGCUGg-CCUGGa-------ACGC- -5' |
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| 24960 | 5' | -55.3 | NC_005284.1 | + | 9998 | 0.77 | 0.171548 |
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Target: 5'- -cGUCGCUGGCCGAgacggaCCGcGGCCUUGCGc -3' miRNA: 3'- aaUAGCGGUUGGCU------GGC-CUGGAACGC- -5' |
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| 24960 | 5' | -55.3 | NC_005284.1 | + | 46862 | 1.06 | 0.001573 |
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Target: 5'- aUUAUCGCCAACCGACCGGACCUUGCGc -3' miRNA: 3'- -AAUAGCGGUUGGCUGGCCUGGAACGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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