Results 1 - 20 of 22 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
24963 | 5' | -53 | NC_005284.1 | + | 44375 | 0.66 | 0.852396 |
Target: 5'- -cGACUGCGcGUC-AUCG-GCcGCCCGc -3' miRNA: 3'- gcUUGACGC-UAGuUAGCuCGaCGGGC- -5' |
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24963 | 5' | -53 | NC_005284.1 | + | 22932 | 0.66 | 0.851538 |
Target: 5'- uCGGGCUGUucuGAUCGAUUGGcgcgcgcGUUGCUCGu -3' miRNA: 3'- -GCUUGACG---CUAGUUAGCU-------CGACGGGC- -5' |
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24963 | 5' | -53 | NC_005284.1 | + | 49164 | 0.66 | 0.838387 |
Target: 5'- aCGGGCccgGCGAggCGAUggaggaaaugcugcgCGAgGCUGCCCGc -3' miRNA: 3'- -GCUUGa--CGCUa-GUUA---------------GCU-CGACGGGC- -5' |
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24963 | 5' | -53 | NC_005284.1 | + | 48959 | 0.66 | 0.834793 |
Target: 5'- gCGGACUGC-AUCGAcgaagCGGuGCUGCgCGg -3' miRNA: 3'- -GCUUGACGcUAGUUa----GCU-CGACGgGC- -5' |
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24963 | 5' | -53 | NC_005284.1 | + | 53158 | 0.66 | 0.810611 |
Target: 5'- uCGAGCuUGCGcgCcauGUCGAGCgcgagcacgcaaucuUGCCCc -3' miRNA: 3'- -GCUUG-ACGCuaGu--UAGCUCG---------------ACGGGc -5' |
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24963 | 5' | -53 | NC_005284.1 | + | 51311 | 0.66 | 0.806771 |
Target: 5'- gGAGCU-CGAgcc--CGAGCUGUCCGa -3' miRNA: 3'- gCUUGAcGCUaguuaGCUCGACGGGC- -5' |
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24963 | 5' | -53 | NC_005284.1 | + | 21391 | 0.66 | 0.806771 |
Target: 5'- -uGACUGCuggCGGUCGAguGCUGCCgGg -3' miRNA: 3'- gcUUGACGcuaGUUAGCU--CGACGGgC- -5' |
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24963 | 5' | -53 | NC_005284.1 | + | 14836 | 0.67 | 0.797039 |
Target: 5'- aCGGaaGCUGcCGAccgugUCGGUCGAGUgGCUCGa -3' miRNA: 3'- -GCU--UGAC-GCU-----AGUUAGCUCGaCGGGC- -5' |
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24963 | 5' | -53 | NC_005284.1 | + | 6893 | 0.67 | 0.78713 |
Target: 5'- gGAACUGCucgcgcauaGcgCGGUUGAGgUGCUCGa -3' miRNA: 3'- gCUUGACG---------CuaGUUAGCUCgACGGGC- -5' |
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24963 | 5' | -53 | NC_005284.1 | + | 32981 | 0.67 | 0.774002 |
Target: 5'- aGGGCgGCGGUCAcucgcuccgcagcgGgCGAugcuGCUGCCCGg -3' miRNA: 3'- gCUUGaCGCUAGU--------------UaGCU----CGACGGGC- -5' |
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24963 | 5' | -53 | NC_005284.1 | + | 11520 | 0.67 | 0.766826 |
Target: 5'- -----cGCGAcCuccUCGAGCUGCCCa -3' miRNA: 3'- gcuugaCGCUaGuu-AGCUCGACGGGc -5' |
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24963 | 5' | -53 | NC_005284.1 | + | 44181 | 0.67 | 0.756455 |
Target: 5'- aCGAA--GCGGUCGAUC-AGUcGCCCGa -3' miRNA: 3'- -GCUUgaCGCUAGUUAGcUCGaCGGGC- -5' |
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24963 | 5' | -53 | NC_005284.1 | + | 29470 | 0.68 | 0.745952 |
Target: 5'- aGGACUGCGGUUAaguaGUUGAcGCagggaacGCCCGa -3' miRNA: 3'- gCUUGACGCUAGU----UAGCU-CGa------CGGGC- -5' |
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24963 | 5' | -53 | NC_005284.1 | + | 22749 | 0.69 | 0.6919 |
Target: 5'- cCGAgcgGCUGCGGUCGAUCaagGGGC-GCgCGa -3' miRNA: 3'- -GCU---UGACGCUAGUUAG---CUCGaCGgGC- -5' |
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24963 | 5' | -53 | NC_005284.1 | + | 36910 | 0.69 | 0.690799 |
Target: 5'- gCGucGCUGCGAUCuuccguuGAUCGAGUucaugaccUGCUCGg -3' miRNA: 3'- -GCu-UGACGCUAG-------UUAGCUCG--------ACGGGC- -5' |
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24963 | 5' | -53 | NC_005284.1 | + | 16922 | 0.69 | 0.680865 |
Target: 5'- uGAGCgcucGC-AUCgGAUCGAGCUGCCgCGc -3' miRNA: 3'- gCUUGa---CGcUAG-UUAGCUCGACGG-GC- -5' |
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24963 | 5' | -53 | NC_005284.1 | + | 44249 | 0.69 | 0.647518 |
Target: 5'- gCGAuccagGCcgGCGAggaaUCGAUCGAgcGCUGCCUGa -3' miRNA: 3'- -GCU-----UGa-CGCU----AGUUAGCU--CGACGGGC- -5' |
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24963 | 5' | -53 | NC_005284.1 | + | 4422 | 0.7 | 0.625195 |
Target: 5'- aCGAGCUGuUGAUCAAgcaGGGCgaacuccaaGCCCGu -3' miRNA: 3'- -GCUUGAC-GCUAGUUag-CUCGa--------CGGGC- -5' |
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24963 | 5' | -53 | NC_005284.1 | + | 45121 | 0.7 | 0.580702 |
Target: 5'- gCGAcGCUGCGAUC-GUCGAGCUGa-UGg -3' miRNA: 3'- -GCU-UGACGCUAGuUAGCUCGACggGC- -5' |
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24963 | 5' | -53 | NC_005284.1 | + | 23328 | 0.72 | 0.50482 |
Target: 5'- gCGGcACgGCGGUCAAUUGGGg-GCCCGg -3' miRNA: 3'- -GCU-UGaCGCUAGUUAGCUCgaCGGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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