Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24965 | 5' | -52 | NC_005284.1 | + | 10414 | 0.7 | 0.625306 |
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Target: 5'- cGGGCGGCCGAugACgcgcagucggcauaUCgGAAUUUGGCGAc -3' miRNA: 3'- -UUCGUCGGCU--UG--------------AGgCUUAAGCUGCU- -5' |
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| 24965 | 5' | -52 | NC_005284.1 | + | 50087 | 0.7 | 0.617374 |
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Target: 5'- cGAGCAGCCGAACagcaUCGAGaacguaacgCGGCGAu -3' miRNA: 3'- -UUCGUCGGCUUGa---GGCUUaa-------GCUGCU- -5' |
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| 24965 | 5' | -52 | NC_005284.1 | + | 22996 | 0.71 | 0.594762 |
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Target: 5'- cGAGcCAGCCGGagccgcGCUCCGAgg-UGACGGa -3' miRNA: 3'- -UUC-GUCGGCU------UGAGGCUuaaGCUGCU- -5' |
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| 24965 | 5' | -52 | NC_005284.1 | + | 23416 | 0.72 | 0.528017 |
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Target: 5'- uGGguGUugaUGAAUUUCGAGUUCGACGAc -3' miRNA: 3'- uUCguCG---GCUUGAGGCUUAAGCUGCU- -5' |
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| 24965 | 5' | -52 | NC_005284.1 | + | 44026 | 0.73 | 0.474607 |
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Target: 5'- aAAGCAGCCGAACgCCc---UCGGCGGc -3' miRNA: 3'- -UUCGUCGGCUUGaGGcuuaAGCUGCU- -5' |
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| 24965 | 5' | -52 | NC_005284.1 | + | 49407 | 1.07 | 0.002667 |
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Target: 5'- cAAGCAGCCGAACUCCGAAUUCGACGAa -3' miRNA: 3'- -UUCGUCGGCUUGAGGCUUAAGCUGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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