Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24966 | 5' | -52.8 | NC_005284.1 | + | 24149 | 0.69 | 0.678211 |
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Target: 5'- -cGCAUgGGGGGCGUGugGuCGGCu-- -3' miRNA: 3'- guCGUAgCUCUUGCAUugC-GCCGcua -5' |
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| 24966 | 5' | -52.8 | NC_005284.1 | + | 38583 | 0.69 | 0.678211 |
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Target: 5'- aCGGCcguUCGGccGAGCGc-ACGCGGCGAg -3' miRNA: 3'- -GUCGu--AGCU--CUUGCauUGCGCCGCUa -5' |
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| 24966 | 5' | -52.8 | NC_005284.1 | + | 40162 | 0.69 | 0.667013 |
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Target: 5'- aCGGCGUCGAG---GUAGCGC-GCGAg -3' miRNA: 3'- -GUCGUAGCUCuugCAUUGCGcCGCUa -5' |
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| 24966 | 5' | -52.8 | NC_005284.1 | + | 27654 | 0.69 | 0.65578 |
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Target: 5'- -uGCAUCGcAGAuGCGU--CGCGGCGGg -3' miRNA: 3'- guCGUAGC-UCU-UGCAuuGCGCCGCUa -5' |
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| 24966 | 5' | -52.8 | NC_005284.1 | + | 17774 | 0.69 | 0.644525 |
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Target: 5'- -cGCGUCGAGGACGagguaaaggcgAACGCcGCGAa -3' miRNA: 3'- guCGUAGCUCUUGCa----------UUGCGcCGCUa -5' |
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| 24966 | 5' | -52.8 | NC_005284.1 | + | 23570 | 0.74 | 0.400271 |
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Target: 5'- -cGCAUCGGcAACGU--CGCGGCGAUg -3' miRNA: 3'- guCGUAGCUcUUGCAuuGCGCCGCUA- -5' |
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| 24966 | 5' | -52.8 | NC_005284.1 | + | 49717 | 0.75 | 0.346514 |
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Target: 5'- gAGCuugugGUCGAGGACGUGAaaucaGCGGUGAc -3' miRNA: 3'- gUCG-----UAGCUCUUGCAUUg----CGCCGCUa -5' |
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| 24966 | 5' | -52.8 | NC_005284.1 | + | 50098 | 1.06 | 0.002882 |
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Target: 5'- aCAGCAUCGAGAACGUAACGCGGCGAUc -3' miRNA: 3'- -GUCGUAGCUCUUGCAUUGCGCCGCUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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