Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
24968 | 5' | -56.5 | NC_005284.1 | + | 457 | 0.66 | 0.645186 |
Target: 5'- gCGAGCcgGCAGcgccuggUCGGCGCaaAGCCGa- -3' miRNA: 3'- -GCUCG--CGUCaa-----AGCCGCGc-UCGGUau -5' |
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24968 | 5' | -56.5 | NC_005284.1 | + | 4773 | 0.67 | 0.546602 |
Target: 5'- uGAGCaagcaGCGGUacgUCguggGGCGCGAGCCu-- -3' miRNA: 3'- gCUCG-----CGUCAa--AG----CCGCGCUCGGuau -5' |
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24968 | 5' | -56.5 | NC_005284.1 | + | 12049 | 0.7 | 0.360663 |
Target: 5'- --cGCGCGGuUUUCGGCGCGugcGGCgAUGu -3' miRNA: 3'- gcuCGCGUC-AAAGCCGCGC---UCGgUAU- -5' |
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24968 | 5' | -56.5 | NC_005284.1 | + | 16122 | 0.68 | 0.48248 |
Target: 5'- -uGGCGCGGUggcgacgUUCGGUuucGCGcAGCCGUGg -3' miRNA: 3'- gcUCGCGUCA-------AAGCCG---CGC-UCGGUAU- -5' |
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24968 | 5' | -56.5 | NC_005284.1 | + | 19402 | 0.68 | 0.492759 |
Target: 5'- aCGAGgGCGGUgcaGGCGUGAucggcgcGCCGUu -3' miRNA: 3'- -GCUCgCGUCAaagCCGCGCU-------CGGUAu -5' |
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24968 | 5' | -56.5 | NC_005284.1 | + | 25668 | 0.74 | 0.229471 |
Target: 5'- -cGGCGCAGccgUUC-GCGCGAGCCAa- -3' miRNA: 3'- gcUCGCGUCa--AAGcCGCGCUCGGUau -5' |
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24968 | 5' | -56.5 | NC_005284.1 | + | 29740 | 0.67 | 0.579149 |
Target: 5'- gGGGCGCcgaacAGcacCGGCGCGAGCgGg- -3' miRNA: 3'- gCUCGCG-----UCaaaGCCGCGCUCGgUau -5' |
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24968 | 5' | -56.5 | NC_005284.1 | + | 36306 | 0.67 | 0.535878 |
Target: 5'- cCGAGCGC-GUUcUCGGCGCGcacCCGc- -3' miRNA: 3'- -GCUCGCGuCAA-AGCCGCGCuc-GGUau -5' |
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24968 | 5' | -56.5 | NC_005284.1 | + | 39497 | 0.66 | 0.609886 |
Target: 5'- gCGGGCGCGGUUggucagugcaugUGGUGCucGCCGc- -3' miRNA: 3'- -GCUCGCGUCAAa-----------GCCGCGcuCGGUau -5' |
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24968 | 5' | -56.5 | NC_005284.1 | + | 43618 | 0.72 | 0.289281 |
Target: 5'- cCGcGCGCGGcggccgCGGCGCGAGCUg-- -3' miRNA: 3'- -GCuCGCGUCaaa---GCCGCGCUCGGuau -5' |
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24968 | 5' | -56.5 | NC_005284.1 | + | 44883 | 0.66 | 0.612089 |
Target: 5'- gCGAGCGCgAGcgacgcUUCGGCGCgcuGAGCUc-- -3' miRNA: 3'- -GCUCGCG-UCa-----AAGCCGCG---CUCGGuau -5' |
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24968 | 5' | -56.5 | NC_005284.1 | + | 51694 | 1.06 | 0.001013 |
Target: 5'- uCGAGCGCAGUUUCGGCGCGAGCCAUAu -3' miRNA: 3'- -GCUCGCGUCAAAGCCGCGCUCGGUAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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