Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
24970 | 5' | -60.8 | NC_005284.1 | + | 53982 | 1.09 | 0.000312 |
Target: 5'- aUGGGGACCACGGCAAAGGCCCGGUGGc -3' miRNA: 3'- -ACCCCUGGUGCCGUUUCCGGGCCACC- -5' |
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24970 | 5' | -60.8 | NC_005284.1 | + | 38679 | 0.67 | 0.395024 |
Target: 5'- aUGGGucgaCGCGGCgAAAGGCUCGaUGGa -3' miRNA: 3'- -ACCCcug-GUGCCG-UUUCCGGGCcACC- -5' |
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24970 | 5' | -60.8 | NC_005284.1 | + | 442 | 0.66 | 0.459097 |
Target: 5'- cUGGGGcucgACC-CGGCGAgccggcAGcGCCUGGUcGGc -3' miRNA: 3'- -ACCCC----UGGuGCCGUU------UC-CGGGCCA-CC- -5' |
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24970 | 5' | -60.8 | NC_005284.1 | + | 29425 | 0.66 | 0.43742 |
Target: 5'- gGGGGGCgGCGGCAAcGGCaaaccacccaugcuaGGcGGg -3' miRNA: 3'- aCCCCUGgUGCCGUUuCCGgg-------------CCaCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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