Results 21 - 32 of 32 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
24971 | 5' | -58 | NC_005284.1 | + | 23496 | 0.7 | 0.352355 |
Target: 5'- aUCCGGCGUGGcGCGCGGaCcuguuccgccuGAUCGcCGa -3' miRNA: 3'- -AGGCUGCGCCaCGCGCC-G-----------UUAGCaGU- -5' |
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24971 | 5' | -58 | NC_005284.1 | + | 16751 | 0.7 | 0.344194 |
Target: 5'- aCUGACuGCGGUGCGCGuGCGcuUCGg-- -3' miRNA: 3'- aGGCUG-CGCCACGCGC-CGUu-AGCagu -5' |
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24971 | 5' | -58 | NC_005284.1 | + | 23351 | 0.7 | 0.33617 |
Target: 5'- cCCGGCGCGccGCaGCGGCGcaCGUCGc -3' miRNA: 3'- aGGCUGCGCcaCG-CGCCGUuaGCAGU- -5' |
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24971 | 5' | -58 | NC_005284.1 | + | 32803 | 0.71 | 0.328283 |
Target: 5'- gCCuGCGCGGguUGCGgGGCGG-CGUCGg -3' miRNA: 3'- aGGcUGCGCC--ACGCgCCGUUaGCAGU- -5' |
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24971 | 5' | -58 | NC_005284.1 | + | 13993 | 0.71 | 0.320535 |
Target: 5'- -aUGGCGCGGcUGCGCGGUAAgcggggCGUg- -3' miRNA: 3'- agGCUGCGCC-ACGCGCCGUUa-----GCAgu -5' |
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24971 | 5' | -58 | NC_005284.1 | + | 14566 | 0.71 | 0.31217 |
Target: 5'- uUUCGGCGCuGUGCGUugcgcuuGGCGAucUCGUCGg -3' miRNA: 3'- -AGGCUGCGcCACGCG-------CCGUU--AGCAGU- -5' |
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24971 | 5' | -58 | NC_005284.1 | + | 13358 | 0.71 | 0.290916 |
Target: 5'- gUCGGCGgcaagcugaCGGUGgGCGGCGAUCuGUCu -3' miRNA: 3'- aGGCUGC---------GCCACgCGCCGUUAG-CAGu -5' |
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24971 | 5' | -58 | NC_005284.1 | + | 43616 | 0.71 | 0.290916 |
Target: 5'- uUCCGcGCGCGGcgGcCGCGGCGcgagcugCGUCAg -3' miRNA: 3'- -AGGC-UGCGCCa-C-GCGCCGUua-----GCAGU- -5' |
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24971 | 5' | -58 | NC_005284.1 | + | 45281 | 0.72 | 0.26613 |
Target: 5'- cUCCG-CGCGGcUGCGCGGCAuggcugaguAgccgaaugcgccuuaUCGUCAc -3' miRNA: 3'- -AGGCuGCGCC-ACGCGCCGU---------U---------------AGCAGU- -5' |
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24971 | 5' | -58 | NC_005284.1 | + | 46100 | 0.76 | 0.148452 |
Target: 5'- gCCGACgauuGCGGUGCGUGGCuccUCGUUu -3' miRNA: 3'- aGGCUG----CGCCACGCGCCGuu-AGCAGu -5' |
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24971 | 5' | -58 | NC_005284.1 | + | 33128 | 0.85 | 0.033527 |
Target: 5'- gCCGACGCGGcggcaucgUGCGCGGCGAUCG-CAc -3' miRNA: 3'- aGGCUGCGCC--------ACGCGCCGUUAGCaGU- -5' |
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24971 | 5' | -58 | NC_005284.1 | + | 54460 | 1.08 | 0.000638 |
Target: 5'- gUCCGACGCGGUGCGCGGCAAUCGUCAu -3' miRNA: 3'- -AGGCUGCGCCACGCGCCGUUAGCAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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