Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24972 | 5' | -57.3 | NC_005284.1 | + | 11850 | 0.66 | 0.573284 |
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Target: 5'- cGGCUCgcgUUGCAGGCcgacuGGCGCGUc -3' miRNA: 3'- -UCGGGacaAACGUUCGuc---CCGCGCAc -5' |
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| 24972 | 5' | -57.3 | NC_005284.1 | + | 29807 | 0.66 | 0.573284 |
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Target: 5'- cAGCCUg----GCAAGUaAGGGUGCGg- -3' miRNA: 3'- -UCGGGacaaaCGUUCG-UCCCGCGCac -5' |
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| 24972 | 5' | -57.3 | NC_005284.1 | + | 31614 | 0.66 | 0.572192 |
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Target: 5'- -cCCUUGggUGCGGGCggcgucgAGGGCGCa-- -3' miRNA: 3'- ucGGGACaaACGUUCG-------UCCCGCGcac -5' |
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| 24972 | 5' | -57.3 | NC_005284.1 | + | 233 | 0.67 | 0.498431 |
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Target: 5'- cGCCCUGcUUGCAAaCAGGGCu---- -3' miRNA: 3'- uCGGGACaAACGUUcGUCCCGcgcac -5' |
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| 24972 | 5' | -57.3 | NC_005284.1 | + | 30904 | 0.67 | 0.477822 |
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Target: 5'- gAGCCCagucgGgaUGCAucgGGC-GGGCGcCGUGg -3' miRNA: 3'- -UCGGGa----CaaACGU---UCGuCCCGC-GCAC- -5' |
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| 24972 | 5' | -57.3 | NC_005284.1 | + | 32542 | 0.67 | 0.477822 |
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Target: 5'- -aCCCUcgcgGCGGGCGGGGCG-GUGu -3' miRNA: 3'- ucGGGAcaaaCGUUCGUCCCGCgCAC- -5' |
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| 24972 | 5' | -57.3 | NC_005284.1 | + | 54362 | 0.67 | 0.463649 |
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Target: 5'- cGCCCgaugaagccgUGCAAGCaccgGGGGUGCGg- -3' miRNA: 3'- uCGGGacaa------ACGUUCG----UCCCGCGCac -5' |
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| 24972 | 5' | -57.3 | NC_005284.1 | + | 21318 | 0.68 | 0.457642 |
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Target: 5'- cGGUUCaaUGcggGCGacuGGCGGGGCGCGUGu -3' miRNA: 3'- -UCGGG--ACaaaCGU---UCGUCCCGCGCAC- -5' |
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| 24972 | 5' | -57.3 | NC_005284.1 | + | 32689 | 0.68 | 0.437928 |
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Target: 5'- cGGCCCgccuacGUagGCAAGCAGGGC-CuUGg -3' miRNA: 3'- -UCGGGa-----CAaaCGUUCGUCCCGcGcAC- -5' |
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| 24972 | 5' | -57.3 | NC_005284.1 | + | 54557 | 1.08 | 0.000539 |
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Target: 5'- cAGCCCUGUUUGCAAGCAGGGCGCGUGa -3' miRNA: 3'- -UCGGGACAAACGUUCGUCCCGCGCAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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