Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2500 | 5' | -59.9 | NC_001454.1 | + | 15121 | 0.67 | 0.259666 |
Target: 5'- gAGCGGCAGuCaUGGCGGCAGUagGCc- -3' miRNA: 3'- -UCGUCGUCuGcGCCGUCGUCGa-CGuc -5' |
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2500 | 5' | -59.9 | NC_001454.1 | + | 11425 | 0.67 | 0.23979 |
Target: 5'- cGGUGGCGGACG-GGUcGCAGCaGguGa -3' miRNA: 3'- -UCGUCGUCUGCgCCGuCGUCGaCguC- -5' |
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2500 | 5' | -59.9 | NC_001454.1 | + | 8161 | 0.68 | 0.225425 |
Target: 5'- cGGUAGCGGcACGUcggcguggagcucgGGUAGCGGUUGguGc -3' miRNA: 3'- -UCGUCGUC-UGCG--------------CCGUCGUCGACguC- -5' |
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2500 | 5' | -59.9 | NC_001454.1 | + | 17482 | 0.68 | 0.2212 |
Target: 5'- gGGCGGCGGGCGgggguagccaCGGCAaCAGUggggGCGGu -3' miRNA: 3'- -UCGUCGUCUGC----------GCCGUcGUCGa---CGUC- -5' |
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2500 | 5' | -59.9 | NC_001454.1 | + | 28146 | 0.68 | 0.2212 |
Target: 5'- gAGcCGGUGGGgGCGGUGGgGGCUGUAa -3' miRNA: 3'- -UC-GUCGUCUgCGCCGUCgUCGACGUc -5' |
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2500 | 5' | -59.9 | NC_001454.1 | + | 9775 | 0.69 | 0.192953 |
Target: 5'- uGGUAGCcaacaaggaAGugG-GGCGGCGGCUcGCGGu -3' miRNA: 3'- -UCGUCG---------UCugCgCCGUCGUCGA-CGUC- -5' |
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2500 | 5' | -59.9 | NC_001454.1 | + | 10535 | 0.69 | 0.182568 |
Target: 5'- cGCAGguuuUAcGCGgGGCAGgAGCUGCGGg -3' miRNA: 3'- uCGUC----GUcUGCgCCGUCgUCGACGUC- -5' |
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2500 | 5' | -59.9 | NC_001454.1 | + | 16373 | 0.69 | 0.182568 |
Target: 5'- cGGCGGCGGACagagcgacgacgGCGGguGgAGCgagUGCGGc -3' miRNA: 3'- -UCGUCGUCUG------------CGCCguCgUCG---ACGUC- -5' |
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2500 | 5' | -59.9 | NC_001454.1 | + | 9894 | 0.7 | 0.154329 |
Target: 5'- gAGcCAGguGAUGcCGGCGGCGGUagugGCGGc -3' miRNA: 3'- -UC-GUCguCUGC-GCCGUCGUCGa---CGUC- -5' |
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2500 | 5' | -59.9 | NC_001454.1 | + | 10346 | 0.7 | 0.141745 |
Target: 5'- uAGUGGgGGGCGgGaGCGGCGGCUGUg- -3' miRNA: 3'- -UCGUCgUCUGCgC-CGUCGUCGACGuc -5' |
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2500 | 5' | -59.9 | NC_001454.1 | + | 6450 | 0.71 | 0.137763 |
Target: 5'- cAGCGGuCGGACcuaGGCGGCAGCUuGCuGg -3' miRNA: 3'- -UCGUC-GUCUGcg-CCGUCGUCGA-CGuC- -5' |
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2500 | 5' | -59.9 | NC_001454.1 | + | 32071 | 0.72 | 0.109437 |
Target: 5'- uGCGGUgcugcgcucgucGGACGCGGCGGCuGCUGg-- -3' miRNA: 3'- uCGUCG------------UCUGCGCCGUCGuCGACguc -5' |
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2500 | 5' | -59.9 | NC_001454.1 | + | 3466 | 1.07 | 0.000176 |
Target: 5'- cAGCAGCAGACGCGGCAGCAGCUGCAGc -3' miRNA: 3'- -UCGUCGUCUGCGCCGUCGUCGACGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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