miRNA: 3'- guUGACGACGA-AGG-GUacUGAguCCU- -5'
miRNA: 3'- gUUGAcGACGAAGGGuacugAGUCCU- -5'
miRNA: 3'- -GUUGACGACGAAGGGUACUGAGUCCU- -5'
Results 1 - 3 of 3 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 25016 | 5' | -52.6 | NC_005301.3 | + | 8258 | 0.66 | 0.390625 |
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Target: 5'- -cACUGCUGCUaUCCuCA--GCUguGGAa -3' miRNA: 3'- guUGACGACGA-AGG-GUacUGAguCCU- -5' |
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| 25016 | 5' | -52.6 | NC_005301.3 | + | 11873 | 0.68 | 0.295582 |
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Target: 5'- gGGCUuCUGCUUCCCua---UCAGGAa -3' miRNA: 3'- gUUGAcGACGAAGGGuacugAGUCCU- -5' |
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| 25016 | 5' | -52.6 | NC_005301.3 | + | 8256 | 1.09 | 0.000173 |
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Target: 5'- aCAACUGCUGCUUCCCAUGACUCAGGAu -3' miRNA: 3'- -GUUGACGACGAAGGGUACUGAGUCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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