Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 25019 | 5' | -50 | NC_005309.1 | + | 204265 | 0.67 | 0.999637 |
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Target: 5'- -gGCCGGaaUUCCGGcCagCuGGAAAUGGu -3' miRNA: 3'- aaUGGUC--AAGGCCuGa-GuCCUUUACC- -5' |
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| 25019 | 5' | -50 | NC_005309.1 | + | 14004 | 0.68 | 0.998994 |
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Target: 5'- gUUACCAGUUCCuGAaUUAGGAAAc-- -3' miRNA: 3'- -AAUGGUCAAGGcCUgAGUCCUUUacc -5' |
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| 25019 | 5' | -50 | NC_005309.1 | + | 156491 | 0.69 | 0.99826 |
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Target: 5'- ---aCGGUUCUGGAguaCUC-GGAGAUGGu -3' miRNA: 3'- aaugGUCAAGGCCU---GAGuCCUUUACC- -5' |
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| 25019 | 5' | -50 | NC_005309.1 | + | 13866 | 0.7 | 0.996625 |
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Target: 5'- gUUACCAGUUCCuGAaUUAGGAAAUu- -3' miRNA: 3'- -AAUGGUCAAGGcCUgAGUCCUUUAcc -5' |
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| 25019 | 5' | -50 | NC_005309.1 | + | 13728 | 1.08 | 0.027563 |
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Target: 5'- gUUACCAGUUCCGGACUCAGGAAAUGGc -3' miRNA: 3'- -AAUGGUCAAGGCCUGAGUCCUUUACC- -5' |
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| 25019 | 5' | -50 | NC_005309.1 | + | 13764 | 1.08 | 0.027563 |
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Target: 5'- gUUACCAGUUCCGGACUCAGGAAAUGGc -3' miRNA: 3'- -AAUGGUCAAGGCCUGAGUCCUUUACC- -5' |
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| 25019 | 5' | -50 | NC_005309.1 | + | 13800 | 1.08 | 0.027563 |
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Target: 5'- gUUACCAGUUCCGGACUCAGGAAAUGGc -3' miRNA: 3'- -AAUGGUCAAGGCCUGAGUCCUUUACC- -5' |
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| 25019 | 5' | -50 | NC_005309.1 | + | 13902 | 1.08 | 0.027563 |
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Target: 5'- gUUACCAGUUCCGGACUCAGGAAAUGGc -3' miRNA: 3'- -AAUGGUCAAGGCCUGAGUCCUUUACC- -5' |
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| 25019 | 5' | -50 | NC_005309.1 | + | 13938 | 1.08 | 0.027563 |
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Target: 5'- gUUACCAGUUCCGGACUCAGGAAAUGGc -3' miRNA: 3'- -AAUGGUCAAGGCCUGAGUCCUUUACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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