Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2502 | 3' | -55.2 | NC_001454.1 | + | 29255 | 0.67 | 0.468254 |
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Target: 5'- --gCCgUCCAcucUCAuUCCuCCCGCAGCGu -3' miRNA: 3'- caaGGgAGGU---AGU-AGGuGGGUGUCGU- -5' |
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| 2502 | 3' | -55.2 | NC_001454.1 | + | 17235 | 0.69 | 0.360703 |
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Target: 5'- gGUUUCCUCCAg-AUCCGgUCGCGGCc -3' miRNA: 3'- -CAAGGGAGGUagUAGGUgGGUGUCGu -5' |
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| 2502 | 3' | -55.2 | NC_001454.1 | + | 31901 | 0.69 | 0.351815 |
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Target: 5'- --aCCgCUCCAUCAagC-CCCGCAGUAa -3' miRNA: 3'- caaGG-GAGGUAGUagGuGGGUGUCGU- -5' |
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| 2502 | 3' | -55.2 | NC_001454.1 | + | 25227 | 0.71 | 0.301793 |
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Target: 5'- -aUCaCUCCGUUcgCuCACCCGCAGCu -3' miRNA: 3'- caAGgGAGGUAGuaG-GUGGGUGUCGu -5' |
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| 2502 | 3' | -55.2 | NC_001454.1 | + | 8167 | 0.72 | 0.257436 |
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Target: 5'- -cUgCCUCCGgaCGcCCGCCCGCAGCGa -3' miRNA: 3'- caAgGGAGGUa-GUaGGUGGGUGUCGU- -5' |
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| 2502 | 3' | -55.2 | NC_001454.1 | + | 7848 | 1.09 | 0.000467 |
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Target: 5'- cGUUCCCUCCAUCAUCCACCCACAGCAa -3' miRNA: 3'- -CAAGGGAGGUAGUAGGUGGGUGUCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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