Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2502 | 5' | -56.7 | NC_001454.1 | + | 16373 | 0.66 | 0.515308 |
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Target: 5'- cGGcgGCGGAcAGAGcGaCGACGGcGGGUGGa -3' miRNA: 3'- -CCa-CGUCU-UCUCcC-GCUGCU-CCCACU- -5' |
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| 2502 | 5' | -56.7 | NC_001454.1 | + | 12637 | 0.67 | 0.452793 |
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Target: 5'- cGUGCcu--GAGGGCGACGAcggauuuuuguGGGaUGAc -3' miRNA: 3'- cCACGucuuCUCCCGCUGCU-----------CCC-ACU- -5' |
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| 2502 | 5' | -56.7 | NC_001454.1 | + | 3773 | 0.67 | 0.423119 |
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Target: 5'- gGGaUGCAGGuaggaccacuGGAGGGCGucAUGcucuGGGGUGGu -3' miRNA: 3'- -CC-ACGUCU----------UCUCCCGC--UGC----UCCCACU- -5' |
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| 2502 | 5' | -56.7 | NC_001454.1 | + | 76 | 0.68 | 0.413489 |
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Target: 5'- aGGUgacGUAGAGGcGGGCGGgGugggaaAGGGUGGa -3' miRNA: 3'- -CCA---CGUCUUCuCCCGCUgC------UCCCACU- -5' |
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| 2502 | 5' | -56.7 | NC_001454.1 | + | 34139 | 0.68 | 0.413489 |
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Target: 5'- aGGUgacGUAGAGGcGGGCGGgGugggaaAGGGUGGa -3' miRNA: 3'- -CCA---CGUCUUCuCCCGCUgC------UCCCACU- -5' |
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| 2502 | 5' | -56.7 | NC_001454.1 | + | 7812 | 1.11 | 0.000282 |
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Target: 5'- aGGUGCAGAAGAGGGCGACGAGGGUGAa -3' miRNA: 3'- -CCACGUCUUCUCCCGCUGCUCCCACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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