Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 25021 | 5' | -45.9 | NC_005309.1 | + | 205024 | 0.66 | 1 |
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Target: 5'- --aGGGGGUGAUUUUAgagguagguacuGUUUCUAa-- -3' miRNA: 3'- cccCCCCCACUAAAAU------------UAAAGGUaua -5' |
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| 25021 | 5' | -45.9 | NC_005309.1 | + | 258165 | 0.71 | 0.999801 |
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Target: 5'- cGGccGGGUGAUUUUAcUUUCCAUAc -3' miRNA: 3'- cCCccCCCACUAAAAUuAAAGGUAUa -5' |
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| 25021 | 5' | -45.9 | NC_005309.1 | + | 252168 | 0.73 | 0.999072 |
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Target: 5'- uGGGGGGcUGAUUUUAucUUCCAa-- -3' miRNA: 3'- cCCCCCCcACUAAAAUuaAAGGUaua -5' |
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| 25021 | 5' | -45.9 | NC_005309.1 | + | 205372 | 0.76 | 0.990768 |
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Target: 5'- --aGGGGGUGAUUUUAcUUUCCAa-- -3' miRNA: 3'- cccCCCCCACUAAAAUuAAAGGUaua -5' |
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| 25021 | 5' | -45.9 | NC_005309.1 | + | 200712 | 0.86 | 0.71721 |
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Target: 5'- uGGGGGGGGUGAUUUUAgaaguagguacuGUUUCUAa-- -3' miRNA: 3'- -CCCCCCCCACUAAAAU------------UAAAGGUaua -5' |
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| 25021 | 5' | -45.9 | NC_005309.1 | + | 201067 | 1.1 | 0.052016 |
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Target: 5'- uGGGGGGGGUGAUUUUAAUUUCCAUAUa -3' miRNA: 3'- -CCCCCCCCACUAAAAUUAAAGGUAUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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