miRNA: 3'- -UCAUGGAUGAAGauuuUAGUGGgcCG- -5'
miRNA: 3'- -UCAUgGAUGAAGAUUUUAGUGggcCG- -5'
miRNA: 3'- -UCAUGGAUGAAGa--------UUUUAGugGGcCG- -5'
miRNA: 3'- -UCAUGGAUGaAGAuuUUAGUGGg--CCG- -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 25022 | 3' | -46.6 | NC_005309.1 | + | 214318 | 0.66 | 1 |
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Target: 5'- cGGUACCUGCUUC----AUCAUCa-GCc -3' miRNA: 3'- -UCAUGGAUGAAGauuuUAGUGGgcCG- -5' |
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| 25022 | 3' | -46.6 | NC_005309.1 | + | 137345 | 0.66 | 1 |
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Target: 5'- uAGUAgCUGCUUCUGGGAUUAUa--GCa -3' miRNA: 3'- -UCAUgGAUGAAGAUUUUAGUGggcCG- -5' |
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| 25022 | 3' | -46.6 | NC_005309.1 | + | 161142 | 0.66 | 1 |
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Target: 5'- cAGUACUUACUUCauuagacgaAGAAUCugCCaGUu -3' miRNA: 3'- -UCAUGGAUGAAGa--------UUUUAGugGGcCG- -5' |
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| 25022 | 3' | -46.6 | NC_005309.1 | + | 143479 | 0.66 | 1 |
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Target: 5'- cAGUAUCUACaUUUuuGAUCAUUacaGGCg -3' miRNA: 3'- -UCAUGGAUGaAGAuuUUAGUGGg--CCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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