Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 25022 | 5' | -47.6 | NC_005309.1 | + | 260338 | 0.67 | 0.999989 |
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Target: 5'- uGcGCgGGGUGAUUUUAUacUCCAa-- -3' miRNA: 3'- gC-CGgCCCACUAAAAUGaaAGGUaug -5' |
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| 25022 | 5' | -47.6 | NC_005309.1 | + | 259206 | 0.67 | 0.999985 |
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Target: 5'- uGGa-GGaccUGGUUUUACUUUCCAUAUa -3' miRNA: 3'- gCCggCCc--ACUAAAAUGAAAGGUAUG- -5' |
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| 25022 | 5' | -47.6 | NC_005309.1 | + | 201066 | 0.73 | 0.994516 |
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Target: 5'- gGGggGGGUGAUUUUAaUUUCCAUAUa -3' miRNA: 3'- gCCggCCCACUAAAAUgAAAGGUAUG- -5' |
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| 25022 | 5' | -47.6 | NC_005309.1 | + | 205372 | 0.75 | 0.98442 |
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Target: 5'- aGG--GGGUGAUUUUACUUUCCAa-- -3' miRNA: 3'- gCCggCCCACUAAAAUGAAAGGUaug -5' |
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| 25022 | 5' | -47.6 | NC_005309.1 | + | 257812 | 0.77 | 0.960649 |
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Target: 5'- cCGGCCGGGUGAUUUuagaaguaggUACUauUUCUAa-- -3' miRNA: 3'- -GCCGGCCCACUAAA----------AUGA--AAGGUaug -5' |
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| 25022 | 5' | -47.6 | NC_005309.1 | + | 251581 | 0.81 | 0.850602 |
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Target: 5'- uGGCCcGGaUGAUUUUACUUUCCAaACg -3' miRNA: 3'- gCCGGcCC-ACUAAAAUGAAAGGUaUG- -5' |
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| 25022 | 5' | -47.6 | NC_005309.1 | + | 197274 | 0.82 | 0.790486 |
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Target: 5'- uGaGCCGGGUGAUUUUAUaUUCCAaACa -3' miRNA: 3'- gC-CGGCCCACUAAAAUGaAAGGUaUG- -5' |
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| 25022 | 5' | -47.6 | NC_005309.1 | + | 258166 | 1.11 | 0.031468 |
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Target: 5'- cCGGCCGGGUGAUUUUACUUUCCAUACu -3' miRNA: 3'- -GCCGGCCCACUAAAAUGAAAGGUAUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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