Results 1 - 20 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
25023 | 3' | -46.6 | NC_005309.1 | + | 174623 | 0.66 | 1 |
Target: 5'- -cUACaUACUUCUAAAAUUAgucuCCCgGCa -3' miRNA: 3'- ucAUGgAUGAAGAUUUUAGU----GGGgCG- -5' |
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25023 | 3' | -46.6 | NC_005309.1 | + | 18697 | 0.67 | 0.999999 |
Target: 5'- cGUAgauCCUACUUCUAccAUUACCUCu- -3' miRNA: 3'- uCAU---GGAUGAAGAUuuUAGUGGGGcg -5' |
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25023 | 3' | -46.6 | NC_005309.1 | + | 191135 | 0.67 | 0.999999 |
Target: 5'- cAGUACUcuUUUCaAGGAcUCGCUCCGCg -3' miRNA: 3'- -UCAUGGauGAAGaUUUU-AGUGGGGCG- -5' |
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25023 | 3' | -46.6 | NC_005309.1 | + | 214318 | 0.68 | 0.999994 |
Target: 5'- cGGUACCUGCUUC-AucAUCAgCCUuCa -3' miRNA: 3'- -UCAUGGAUGAAGaUuuUAGUgGGGcG- -5' |
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25023 | 3' | -46.6 | NC_005309.1 | + | 135489 | 0.68 | 0.999989 |
Target: 5'- --gGCC-ACUUCUaGAGAUCACUgUGCg -3' miRNA: 3'- ucaUGGaUGAAGA-UUUUAGUGGgGCG- -5' |
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25023 | 3' | -46.6 | NC_005309.1 | + | 137345 | 0.69 | 0.999979 |
Target: 5'- uAGUAgCUGCUUCUGGGAUUAUa--GCa -3' miRNA: 3'- -UCAUgGAUGAAGAUUUUAGUGgggCG- -5' |
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25023 | 3' | -46.6 | NC_005309.1 | + | 237963 | 0.69 | 0.999972 |
Target: 5'- cAGUACCUGgUUCUAAA---GCCgCGUu -3' miRNA: 3'- -UCAUGGAUgAAGAUUUuagUGGgGCG- -5' |
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25023 | 3' | -46.6 | NC_005309.1 | + | 354342 | 0.69 | 0.999951 |
Target: 5'- uGGUGCCguUGCUUCUG----UugCCCGUg -3' miRNA: 3'- -UCAUGG--AUGAAGAUuuuaGugGGGCG- -5' |
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25023 | 3' | -46.6 | NC_005309.1 | + | 5512 | 0.69 | 0.999951 |
Target: 5'- uGGUGCCguUGCUUCUG----UugCCCGUg -3' miRNA: 3'- -UCAUGG--AUGAAGAUuuuaGugGGGCG- -5' |
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25023 | 3' | -46.6 | NC_005309.1 | + | 23112 | 0.7 | 0.999935 |
Target: 5'- cGUACCUAUUUaUUGAGuuuacAUCAgCCCCGUu -3' miRNA: 3'- uCAUGGAUGAA-GAUUU-----UAGU-GGGGCG- -5' |
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25023 | 3' | -46.6 | NC_005309.1 | + | 141455 | 0.7 | 0.99989 |
Target: 5'- --cGCCUGCUaCcAAAAUCAUCCUGUu -3' miRNA: 3'- ucaUGGAUGAaGaUUUUAGUGGGGCG- -5' |
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25023 | 3' | -46.6 | NC_005309.1 | + | 347899 | 0.7 | 0.999855 |
Target: 5'- -aUACCUACUUCUAucguaugGAAUCGCUauugaCGUa -3' miRNA: 3'- ucAUGGAUGAAGAU-------UUUAGUGGg----GCG- -5' |
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25023 | 3' | -46.6 | NC_005309.1 | + | 10175 | 0.71 | 0.999819 |
Target: 5'- gAGUcCCUACUcgUUUuacGUCGCUCCGCg -3' miRNA: 3'- -UCAuGGAUGA--AGAuuuUAGUGGGGCG- -5' |
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25023 | 3' | -46.6 | NC_005309.1 | + | 259946 | 0.71 | 0.999637 |
Target: 5'- aAGUAa-UAUUUggaguaUAAAAUCACCCCGCg -3' miRNA: 3'- -UCAUggAUGAAg-----AUUUUAGUGGGGCG- -5' |
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25023 | 3' | -46.6 | NC_005309.1 | + | 5692 | 0.72 | 0.999311 |
Target: 5'- uGUAUCUGgaUCUG--GUCACCCUGUu -3' miRNA: 3'- uCAUGGAUgaAGAUuuUAGUGGGGCG- -5' |
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25023 | 3' | -46.6 | NC_005309.1 | + | 354162 | 0.72 | 0.999311 |
Target: 5'- uGUAUCUGgaUCUG--GUCACCCUGUu -3' miRNA: 3'- uCAUGGAUgaAGAUuuUAGUGGGGCG- -5' |
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25023 | 3' | -46.6 | NC_005309.1 | + | 253312 | 0.72 | 0.999311 |
Target: 5'- --cAUUUACUUCUAAAAUCAUUuuGCc -3' miRNA: 3'- ucaUGGAUGAAGAUUUUAGUGGggCG- -5' |
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25023 | 3' | -46.6 | NC_005309.1 | + | 282773 | 0.73 | 0.998504 |
Target: 5'- cGGUAUCUACUUCUGAAAcCAUgCaGCg -3' miRNA: 3'- -UCAUGGAUGAAGAUUUUaGUGgGgCG- -5' |
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25023 | 3' | -46.6 | NC_005309.1 | + | 205409 | 0.92 | 0.395274 |
Target: 5'- cAGUACCUACcUCUAAAAUCACCCCc- -3' miRNA: 3'- -UCAUGGAUGaAGAUUUUAGUGGGGcg -5' |
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25023 | 3' | -46.6 | NC_005309.1 | + | 197307 | 0.93 | 0.370151 |
Target: 5'- uGUACCcACUUCUAAAAUCACCCgGCu -3' miRNA: 3'- uCAUGGaUGAAGAUUUUAGUGGGgCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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