Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 25024 | 3' | -45.2 | NC_005309.1 | + | 200681 | 0.67 | 1 |
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Target: 5'- --aUAUGGAAAuUAAAAUCACCCc-- -3' miRNA: 3'- uucAUACCUUUcAUUUUAGUGGGccg -5' |
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| 25024 | 3' | -45.2 | NC_005309.1 | + | 259945 | 0.71 | 0.999966 |
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Target: 5'- uAAGUAauauuUGGAGuaUAAAAUCACCCcGCg -3' miRNA: 3'- -UUCAU-----ACCUUucAUUUUAGUGGGcCG- -5' |
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| 25024 | 3' | -45.2 | NC_005309.1 | + | 6193 | 0.72 | 0.999867 |
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Target: 5'- ----cUGGAGAGUuccGAGGguUCAUCCGGCa -3' miRNA: 3'- uucauACCUUUCA---UUUU--AGUGGGCCG- -5' |
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| 25024 | 3' | -45.2 | NC_005309.1 | + | 353661 | 0.72 | 0.999867 |
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Target: 5'- ----cUGGAGAGUuccGAGGguUCAUCCGGCa -3' miRNA: 3'- uucauACCUUUCA---UUUU--AGUGGGCCG- -5' |
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| 25024 | 3' | -45.2 | NC_005309.1 | + | 204992 | 0.75 | 0.99643 |
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Target: 5'- ----uUGGAAAGUAAAAUCACCCc-- -3' miRNA: 3'- uucauACCUUUCAUUUUAGUGGGccg -5' |
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| 25024 | 3' | -45.2 | NC_005309.1 | + | 252893 | 0.76 | 0.994316 |
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Target: 5'- gAAGUGUGGAAAaUAAAAUCACuaggacagaauuCUGGCa -3' miRNA: 3'- -UUCAUACCUUUcAUUUUAGUG------------GGCCG- -5' |
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| 25024 | 3' | -45.2 | NC_005309.1 | + | 196885 | 0.84 | 0.823794 |
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Target: 5'- cAGUGuuUGGAAuaUAAAAUCACCCGGCu -3' miRNA: 3'- uUCAU--ACCUUucAUUUUAGUGGGCCG- -5' |
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| 25024 | 3' | -45.2 | NC_005309.1 | + | 251195 | 0.85 | 0.777737 |
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Target: 5'- -cGUuUGGAAAGUAAAAUCAUCCGGg -3' miRNA: 3'- uuCAuACCUUUCAUUUUAGUGGGCCg -5' |
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| 25024 | 3' | -45.2 | NC_005309.1 | + | 257778 | 1.1 | 0.056918 |
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Target: 5'- aAAGUAUGGAAAGUAAAAUCACCCGGCc -3' miRNA: 3'- -UUCAUACCUUUCAUUUUAGUGGGCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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