Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 25024 | 5' | -49.9 | NC_005309.1 | + | 258166 | 0.68 | 0.99919 |
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Target: 5'- cCGGCCGGGUGAUUUUAc--------- -3' miRNA: 3'- -GCCGGCCCACUAAAAUcuucauccau -5' |
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| 25024 | 5' | -49.9 | NC_005309.1 | + | 205024 | 0.81 | 0.661994 |
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Target: 5'- aGG--GGGUGAUUUUAGAGGUAGGUAc -3' miRNA: 3'- gCCggCCCACUAAAAUCUUCAUCCAU- -5' |
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| 25024 | 5' | -49.9 | NC_005309.1 | + | 200713 | 0.86 | 0.454845 |
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Target: 5'- gGGggGGGUGAUUUUAGAAGUAGGUAc -3' miRNA: 3'- gCCggCCCACUAAAAUCUUCAUCCAU- -5' |
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| 25024 | 5' | -49.9 | NC_005309.1 | + | 259984 | 0.9 | 0.287752 |
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Target: 5'- uGcGCgGGGUGAUUUUAGAAGUAGGUAc -3' miRNA: 3'- gC-CGgCCCACUAAAAUCUUCAUCCAU- -5' |
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| 25024 | 5' | -49.9 | NC_005309.1 | + | 196920 | 0.97 | 0.114766 |
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Target: 5'- uGaGCCGGGUGAUUUUAGAAGUGGGUAc -3' miRNA: 3'- gC-CGGCCCACUAAAAUCUUCAUCCAU- -5' |
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| 25024 | 5' | -49.9 | NC_005309.1 | + | 257812 | 1.08 | 0.029292 |
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Target: 5'- cCGGCCGGGUGAUUUUAGAAGUAGGUAc -3' miRNA: 3'- -GCCGGCCCACUAAAAUCUUCAUCCAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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