Results 1 - 18 of 18 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
25026 | 3' | -57.7 | NC_005336.1 | + | 138888 | 0.66 | 0.828525 |
Target: 5'- uUUCGGAGGAGc-GcGGAG-GGCGGGu -3' miRNA: 3'- -AAGCCUCCUCucCuCCUCuUCGCCUc -5' |
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25026 | 3' | -57.7 | NC_005336.1 | + | 139104 | 0.66 | 0.828525 |
Target: 5'- uUUCGGAGGAGc-GcGGAG-GGCGGGu -3' miRNA: 3'- -AAGCCUCCUCucCuCCUCuUCGCCUc -5' |
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25026 | 3' | -57.7 | NC_005336.1 | + | 139104 | 0.66 | 0.828525 |
Target: 5'- uUUCGGAGGAGc-GcGGAG-GGCGGGu -3' miRNA: 3'- -AAGCCUCCUCucCuCCUCuUCGCCUc -5' |
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25026 | 3' | -57.7 | NC_005336.1 | + | 138888 | 0.66 | 0.828525 |
Target: 5'- uUUCGGAGGAGc-GcGGAG-GGCGGGu -3' miRNA: 3'- -AAGCCUCCUCucCuCCUCuUCGCCUc -5' |
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25026 | 3' | -57.7 | NC_005336.1 | + | 32756 | 0.67 | 0.765587 |
Target: 5'- -gUGGuGGAGAGGA--AGGAGCGGuGc -3' miRNA: 3'- aaGCCuCCUCUCCUccUCUUCGCCuC- -5' |
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25026 | 3' | -57.7 | NC_005336.1 | + | 25214 | 0.67 | 0.762738 |
Target: 5'- cUCGGGGGAGAgucccaccuccgcuGGuGGAcGuuGUGGAGa -3' miRNA: 3'- aAGCCUCCUCU--------------CCuCCU-CuuCGCCUC- -5' |
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25026 | 3' | -57.7 | NC_005336.1 | + | 12843 | 0.67 | 0.756048 |
Target: 5'- --aGGAGuuGAGGGGGAGAaaaacAGCGGu- -3' miRNA: 3'- aagCCUCcuCUCCUCCUCU-----UCGCCuc -5' |
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25026 | 3' | -57.7 | NC_005336.1 | + | 32686 | 0.67 | 0.756048 |
Target: 5'- gUCGGGGGugacgauGAGGAuGcAGGAGCuGGAGa -3' miRNA: 3'- aAGCCUCCu------CUCCUcC-UCUUCG-CCUC- -5' |
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25026 | 3' | -57.7 | NC_005336.1 | + | 13276 | 0.67 | 0.756048 |
Target: 5'- -cCGGGGGAuGuGGGGGuuucgAGAAgcGCGGAGa -3' miRNA: 3'- aaGCCUCCU-CuCCUCC-----UCUU--CGCCUC- -5' |
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25026 | 3' | -57.7 | NC_005336.1 | + | 18596 | 0.67 | 0.735669 |
Target: 5'- gUCGGAGGAGuGucugcucuuuuccGAGGGGcAGCGGu- -3' miRNA: 3'- aAGCCUCCUCuC-------------CUCCUCuUCGCCuc -5' |
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25026 | 3' | -57.7 | NC_005336.1 | + | 23610 | 0.67 | 0.726808 |
Target: 5'- aUUUGGAGGGcGGGGuGGGGu--CGGAGg -3' miRNA: 3'- -AAGCCUCCU-CUCCuCCUCuucGCCUC- -5' |
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25026 | 3' | -57.7 | NC_005336.1 | + | 93438 | 0.69 | 0.63564 |
Target: 5'- -cUGGAGGAGgcgcggcggcGGGAGcuAGAAGCGGAa -3' miRNA: 3'- aaGCCUCCUC----------UCCUCc-UCUUCGCCUc -5' |
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25026 | 3' | -57.7 | NC_005336.1 | + | 114865 | 0.69 | 0.615132 |
Target: 5'- -cCGGuGGAGAagGcGAGGAGGAG-GGAGa -3' miRNA: 3'- aaGCCuCCUCU--C-CUCCUCUUCgCCUC- -5' |
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25026 | 3' | -57.7 | NC_005336.1 | + | 92720 | 0.7 | 0.574329 |
Target: 5'- -aCGGAGGAGAuGGGGaucuugaacGAGAAGCGcucGAGg -3' miRNA: 3'- aaGCCUCCUCU-CCUC---------CUCUUCGC---CUC- -5' |
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25026 | 3' | -57.7 | NC_005336.1 | + | 3 | 0.71 | 0.503761 |
Target: 5'- -gCGGAccaGGAGuuccugcGGGAGGAGuuacAGCGGAGg -3' miRNA: 3'- aaGCCU---CCUC-------UCCUCCUCu---UCGCCUC- -5' |
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25026 | 3' | -57.7 | NC_005336.1 | + | 3 | 0.71 | 0.503761 |
Target: 5'- -gCGGAccaGGAGuuccugcGGGAGGAGuuacAGCGGAGg -3' miRNA: 3'- aaGCCU---CCUC-------UCCUCCUCu---UCGCCUC- -5' |
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25026 | 3' | -57.7 | NC_005336.1 | + | 138989 | 1.06 | 0.002809 |
Target: 5'- uUUCGGAGGAGAGGAGGAGAAGCGGAGu -3' miRNA: 3'- -AAGCCUCCUCUCCUCCUCUUCGCCUC- -5' |
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25026 | 3' | -57.7 | NC_005336.1 | + | 138989 | 1.06 | 0.002809 |
Target: 5'- uUUCGGAGGAGAGGAGGAGAAGCGGAGu -3' miRNA: 3'- -AAGCCUCCUCUCCUCCUCUUCGCCUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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