Results 1 - 20 of 37 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
25034 | 3' | -54.5 | NC_005336.1 | + | 18586 | 0.66 | 0.956071 |
Target: 5'- aGCGGu-GCCUgUCGGaGgagUGUCUGCUc -3' miRNA: 3'- gUGCCuuUGGGaAGCC-Ca--ACAGGCGG- -5' |
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25034 | 3' | -54.5 | NC_005336.1 | + | 34348 | 0.66 | 0.956071 |
Target: 5'- gGCGaGAAGCCCUgguggUGGGgccagCaCGCCg -3' miRNA: 3'- gUGC-CUUUGGGAa----GCCCaaca-G-GCGG- -5' |
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25034 | 3' | -54.5 | NC_005336.1 | + | 50520 | 0.66 | 0.956071 |
Target: 5'- gGCGGAAGgCCUgUCGGGacg--CGCCg -3' miRNA: 3'- gUGCCUUUgGGA-AGCCCaacagGCGG- -5' |
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25034 | 3' | -54.5 | NC_005336.1 | + | 97576 | 0.66 | 0.952121 |
Target: 5'- cCACGGcgGCCgCggugcgCGGGUa--CCGCCg -3' miRNA: 3'- -GUGCCuuUGG-Gaa----GCCCAacaGGCGG- -5' |
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25034 | 3' | -54.5 | NC_005336.1 | + | 25079 | 0.66 | 0.952121 |
Target: 5'- -cCGGc-GCCUgaCGGGgagGUCCGCUg -3' miRNA: 3'- guGCCuuUGGGaaGCCCaa-CAGGCGG- -5' |
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25034 | 3' | -54.5 | NC_005336.1 | + | 27254 | 0.66 | 0.952121 |
Target: 5'- aCGCGGuagcACCCgUCuGGGUcgaUGUCaugCGCCa -3' miRNA: 3'- -GUGCCuu--UGGGaAG-CCCA---ACAG---GCGG- -5' |
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25034 | 3' | -54.5 | NC_005336.1 | + | 6053 | 0.66 | 0.952121 |
Target: 5'- aCGCGcGGACCacggUCGcGUaGUCCGCCa -3' miRNA: 3'- -GUGCcUUUGGga--AGCcCAaCAGGCGG- -5' |
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25034 | 3' | -54.5 | NC_005336.1 | + | 60707 | 0.66 | 0.947939 |
Target: 5'- cCGCGGAGauagucgagguGCCCccgUCGGGcgucgUGUCCauCCa -3' miRNA: 3'- -GUGCCUU-----------UGGGa--AGCCCa----ACAGGc-GG- -5' |
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25034 | 3' | -54.5 | NC_005336.1 | + | 39833 | 0.66 | 0.947939 |
Target: 5'- gCGCGGAGugcaagguGCUCUUCGGGcccUUCGCg -3' miRNA: 3'- -GUGCCUU--------UGGGAAGCCCaacAGGCGg -5' |
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25034 | 3' | -54.5 | NC_005336.1 | + | 110662 | 0.66 | 0.943522 |
Target: 5'- uCACGGGAgaGCCCUcgagCGcGGccaccgCCGCCa -3' miRNA: 3'- -GUGCCUU--UGGGAa---GC-CCaaca--GGCGG- -5' |
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25034 | 3' | -54.5 | NC_005336.1 | + | 135815 | 0.66 | 0.943522 |
Target: 5'- gGCGGcGGGCCCggacgcggCGGGgcGgCUGCCg -3' miRNA: 3'- gUGCC-UUUGGGaa------GCCCaaCaGGCGG- -5' |
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25034 | 3' | -54.5 | NC_005336.1 | + | 30706 | 0.66 | 0.938868 |
Target: 5'- gGCGGuggcGGCCUcggagUUCGGGU--UCCGCUc -3' miRNA: 3'- gUGCCu---UUGGG-----AAGCCCAacAGGCGG- -5' |
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25034 | 3' | -54.5 | NC_005336.1 | + | 88791 | 0.66 | 0.938389 |
Target: 5'- aGCGGcguGGACCCgcugcggaagaugaCGGGUUGgugaugcgccggCCGCCg -3' miRNA: 3'- gUGCC---UUUGGGaa------------GCCCAACa-----------GGCGG- -5' |
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25034 | 3' | -54.5 | NC_005336.1 | + | 101344 | 0.66 | 0.933975 |
Target: 5'- uCGCGGAcaAGCUCUUCGGGaccuaCGCg -3' miRNA: 3'- -GUGCCU--UUGGGAAGCCCaacagGCGg -5' |
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25034 | 3' | -54.5 | NC_005336.1 | + | 68415 | 0.66 | 0.933975 |
Target: 5'- -uCGGGGACCgCgUCGGGcgUGggCGCCa -3' miRNA: 3'- guGCCUUUGG-GaAGCCCa-ACagGCGG- -5' |
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25034 | 3' | -54.5 | NC_005336.1 | + | 25438 | 0.67 | 0.930406 |
Target: 5'- gCGCGGccggcgaAGGCCUcgucgcgcagcagCGGGUUGUCCGgCg -3' miRNA: 3'- -GUGCC-------UUUGGGaa-----------GCCCAACAGGCgG- -5' |
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25034 | 3' | -54.5 | NC_005336.1 | + | 126718 | 0.67 | 0.92884 |
Target: 5'- aCGCGGcgccGCCUgcaccgcgcucUCGGGUUGgggCCGUCg -3' miRNA: 3'- -GUGCCuu--UGGGa----------AGCCCAACa--GGCGG- -5' |
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25034 | 3' | -54.5 | NC_005336.1 | + | 71647 | 0.67 | 0.923465 |
Target: 5'- cCACGGAGGCCCaggcgUUCGcGGcg--CCGCg -3' miRNA: 3'- -GUGCCUUUGGG-----AAGC-CCaacaGGCGg -5' |
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25034 | 3' | -54.5 | NC_005336.1 | + | 78478 | 0.67 | 0.917849 |
Target: 5'- gCGCGGuacguACaugagCGGGUUGUgCGCCu -3' miRNA: 3'- -GUGCCuu---UGggaa-GCCCAACAgGCGG- -5' |
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25034 | 3' | -54.5 | NC_005336.1 | + | 100543 | 0.68 | 0.899565 |
Target: 5'- -gUGGAAACCagcaccgCGGGccgGUCCGCg -3' miRNA: 3'- guGCCUUUGGgaa----GCCCaa-CAGGCGg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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