Results 1 - 17 of 17 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
25036 | 5' | -53.6 | NC_005336.1 | + | 131919 | 0.66 | 0.960252 |
Target: 5'- gCCCAGc---GAGGCCucg-CGGCUCg -3' miRNA: 3'- aGGGUCacauCUCCGGuuaaGUCGGG- -5' |
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25036 | 5' | -53.6 | NC_005336.1 | + | 131015 | 0.66 | 0.960252 |
Target: 5'- cUuuGGUGUGGccGuCCAAggaugUCAGCCCu -3' miRNA: 3'- aGggUCACAUCucC-GGUUa----AGUCGGG- -5' |
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25036 | 5' | -53.6 | NC_005336.1 | + | 74444 | 0.66 | 0.960252 |
Target: 5'- gCUCGGUGgAGuGGCgCAGcagaCAGCCCu -3' miRNA: 3'- aGGGUCACaUCuCCG-GUUaa--GUCGGG- -5' |
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25036 | 5' | -53.6 | NC_005336.1 | + | 110970 | 0.66 | 0.956473 |
Target: 5'- gCUCGGUGUGcuGGGGCCcuUUgAGCUg -3' miRNA: 3'- aGGGUCACAU--CUCCGGuuAAgUCGGg -5' |
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25036 | 5' | -53.6 | NC_005336.1 | + | 32840 | 0.66 | 0.956082 |
Target: 5'- gCCCAGaUGUcuaggccGGAGGCUAucaugCAGCgCa -3' miRNA: 3'- aGGGUC-ACA-------UCUCCGGUuaa--GUCGgG- -5' |
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25036 | 5' | -53.6 | NC_005336.1 | + | 19940 | 0.66 | 0.952456 |
Target: 5'- --gCGGUGUGGcGcGCCAGUUUcugcuGGCCCc -3' miRNA: 3'- aggGUCACAUCuC-CGGUUAAG-----UCGGG- -5' |
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25036 | 5' | -53.6 | NC_005336.1 | + | 83120 | 0.66 | 0.952456 |
Target: 5'- -gCCAGUcGUgcauGGAGGUCAug-CGGUCCa -3' miRNA: 3'- agGGUCA-CA----UCUCCGGUuaaGUCGGG- -5' |
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25036 | 5' | -53.6 | NC_005336.1 | + | 101050 | 0.66 | 0.943693 |
Target: 5'- -gCCGGUG-AGuGGCCAccacgAGCCCg -3' miRNA: 3'- agGGUCACaUCuCCGGUuaag-UCGGG- -5' |
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25036 | 5' | -53.6 | NC_005336.1 | + | 95643 | 0.68 | 0.905153 |
Target: 5'- gCCCAGacccGUGGccGCCGGcagcgcgUCGGCCCa -3' miRNA: 3'- aGGGUCa---CAUCucCGGUUa------AGUCGGG- -5' |
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25036 | 5' | -53.6 | NC_005336.1 | + | 11600 | 0.68 | 0.898647 |
Target: 5'- uUCCCGGUGUccGAGaGCCAGaagCuGCCg -3' miRNA: 3'- -AGGGUCACAu-CUC-CGGUUaa-GuCGGg -5' |
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25036 | 5' | -53.6 | NC_005336.1 | + | 133116 | 0.68 | 0.891898 |
Target: 5'- cCUCGGUGgAGAuGGCCAAga-AGCCg -3' miRNA: 3'- aGGGUCACaUCU-CCGGUUaagUCGGg -5' |
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25036 | 5' | -53.6 | NC_005336.1 | + | 43526 | 0.69 | 0.870225 |
Target: 5'- gCCCGGUGUAcgcGGGcGCCAAgUCGGaggaCCu -3' miRNA: 3'- aGGGUCACAU---CUC-CGGUUaAGUCg---GG- -5' |
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25036 | 5' | -53.6 | NC_005336.1 | + | 44870 | 0.69 | 0.854642 |
Target: 5'- gUCCCGGc--GGAGGCCGugacggAGCCCu -3' miRNA: 3'- -AGGGUCacaUCUCCGGUuaag--UCGGG- -5' |
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25036 | 5' | -53.6 | NC_005336.1 | + | 65895 | 0.71 | 0.735596 |
Target: 5'- cUCUGGUGc-GAGGCCGug-CGGCCCg -3' miRNA: 3'- aGGGUCACauCUCCGGUuaaGUCGGG- -5' |
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25036 | 5' | -53.6 | NC_005336.1 | + | 59681 | 0.71 | 0.735596 |
Target: 5'- cCUCGGUG-AGcAGGCCGcugcgCAGCCCu -3' miRNA: 3'- aGGGUCACaUC-UCCGGUuaa--GUCGGG- -5' |
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25036 | 5' | -53.6 | NC_005336.1 | + | 79618 | 0.76 | 0.498212 |
Target: 5'- cUCCCGGUGgAGAGGUCG-UUgAGCUCg -3' miRNA: 3'- -AGGGUCACaUCUCCGGUuAAgUCGGG- -5' |
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25036 | 5' | -53.6 | NC_005336.1 | + | 132349 | 1.13 | 0.002087 |
Target: 5'- gUCCCAGUGUAGAGGCCAAUUCAGCCCg -3' miRNA: 3'- -AGGGUCACAUCUCCGGUUAAGUCGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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