miRNA: 3'- uCACAAAGGCGGCagguagCUGGUCC- -5'
miRNA: 3'- ucaCAAAGGCGGCa--GGUAGCUGGU-CC- -5'
miRNA: 3'- -UCACAAAGGCGGCAGGUAGCUgGUCC- -5'
Results 21 - 23 of 23 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 25037 | 3' | -54 | NC_005336.1 | + | 40258 | 0.71 | 0.791377 |
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Target: 5'- cGUGUUUCUGCCGaa----GACCAGGu -3' miRNA: 3'- uCACAAAGGCGGCagguagCUGGUCC- -5' |
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| 25037 | 3' | -54 | NC_005336.1 | + | 19955 | 0.72 | 0.724205 |
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Target: 5'- --aGUUUCUGCUGgccCCGUCGGCCGcGGc -3' miRNA: 3'- ucaCAAAGGCGGCa--GGUAGCUGGU-CC- -5' |
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| 25037 | 3' | -54 | NC_005336.1 | + | 131715 | 0.98 | 0.024477 |
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Target: 5'- aAGUGUUUCCGCCGUCCAUCGA-CAGGa -3' miRNA: 3'- -UCACAAAGGCGGCAGGUAGCUgGUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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