Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 25037 | 5' | -56.5 | NC_005336.1 | + | 117322 | 0.67 | 0.805157 |
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Target: 5'- uGUCgCUgaagaGGUCGCggaAGCGcucGGCGGGGACg -3' miRNA: 3'- -CAG-GA-----UCAGCGg--UUGCa--CCGCCUCUG- -5' |
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| 25037 | 5' | -56.5 | NC_005336.1 | + | 123500 | 0.67 | 0.838621 |
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Target: 5'- gGUCgCUAGUCucggacgcgcgGCCcgugacuGGCGUGGUGGAGcGCg -3' miRNA: 3'- -CAG-GAUCAG-----------CGG-------UUGCACCGCCUC-UG- -5' |
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| 25037 | 5' | -56.5 | NC_005336.1 | + | 123615 | 0.71 | 0.576735 |
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Target: 5'- aGUCCgUGGgCGCCAACGUGGCGa---- -3' miRNA: 3'- -CAGG-AUCaGCGGUUGCACCGCcucug -5' |
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| 25037 | 5' | -56.5 | NC_005336.1 | + | 125065 | 0.67 | 0.796169 |
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Target: 5'- cUCgcGGUCGCCggUGUGGCuGGcGGCa -3' miRNA: 3'- cAGgaUCAGCGGuuGCACCG-CCuCUG- -5' |
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| 25037 | 5' | -56.5 | NC_005336.1 | + | 125351 | 0.68 | 0.767394 |
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Target: 5'- cGUCCgcacGUCGCgCGaaccgcaguguggACGcUGGCGGAGAUu -3' miRNA: 3'- -CAGGau--CAGCG-GU-------------UGC-ACCGCCUCUG- -5' |
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| 25037 | 5' | -56.5 | NC_005336.1 | + | 128105 | 0.66 | 0.885044 |
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Target: 5'- aUCCg---CGCCuGCGaGGCGGAGGu -3' miRNA: 3'- cAGGaucaGCGGuUGCaCCGCCUCUg -5' |
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| 25037 | 5' | -56.5 | NC_005336.1 | + | 129364 | 0.66 | 0.863189 |
|
Target: 5'- uUCCUGGacauccugCGgCAGCGc-GCGGAGACg -3' miRNA: 3'- cAGGAUCa-------GCgGUUGCacCGCCUCUG- -5' |
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| 25037 | 5' | -56.5 | NC_005336.1 | + | 131748 | 1.1 | 0.001935 |
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Target: 5'- aGUCCUAGUCGCCAACGUGGCGGAGACg -3' miRNA: 3'- -CAGGAUCAGCGGUUGCACCGCCUCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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