Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 25037 | 5' | -56.5 | NC_005336.1 | + | 103549 | 0.68 | 0.749164 |
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Target: 5'- cUCgUGGUCGagaaCGGCGUGcucgucauggacGCGGAGGCg -3' miRNA: 3'- cAGgAUCAGCg---GUUGCAC------------CGCCUCUG- -5' |
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| 25037 | 5' | -56.5 | NC_005336.1 | + | 114525 | 0.69 | 0.693493 |
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Target: 5'- -cCCUAGUCGCUgggacagcgacagcgAGgGUGaCGGAGACa -3' miRNA: 3'- caGGAUCAGCGG---------------UUgCACcGCCUCUG- -5' |
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| 25037 | 5' | -56.5 | NC_005336.1 | + | 65141 | 0.7 | 0.669042 |
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Target: 5'- -cCCUGGcCGCCAcgcuGCGccUGGCcucGGAGACg -3' miRNA: 3'- caGGAUCaGCGGU----UGC--ACCG---CCUCUG- -5' |
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| 25037 | 5' | -56.5 | NC_005336.1 | + | 56822 | 0.71 | 0.583867 |
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Target: 5'- uUCCUGGUcuucgugaaaccggCGCCcuCGgaGGCGGAGGCg -3' miRNA: 3'- cAGGAUCA--------------GCGGuuGCa-CCGCCUCUG- -5' |
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| 25037 | 5' | -56.5 | NC_005336.1 | + | 123615 | 0.71 | 0.576735 |
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Target: 5'- aGUCCgUGGgCGCCAACGUGGCGa---- -3' miRNA: 3'- -CAGG-AUCaGCGGUUGCACCGCcucug -5' |
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| 25037 | 5' | -56.5 | NC_005336.1 | + | 105526 | 0.72 | 0.546418 |
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Target: 5'- -cCCcAGUCGCCGcgcGCGUGcGCcGGGGGCa -3' miRNA: 3'- caGGaUCAGCGGU---UGCAC-CG-CCUCUG- -5' |
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| 25037 | 5' | -56.5 | NC_005336.1 | + | 58988 | 0.77 | 0.296294 |
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Target: 5'- gGUCCUGG-CGCCcGC-UGGUGGAGGCu -3' miRNA: 3'- -CAGGAUCaGCGGuUGcACCGCCUCUG- -5' |
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| 25037 | 5' | -56.5 | NC_005336.1 | + | 131748 | 1.1 | 0.001935 |
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Target: 5'- aGUCCUAGUCGCCAACGUGGCGGAGACg -3' miRNA: 3'- -CAGGAUCAGCGGUUGCACCGCCUCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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