Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 25046 | 5' | -57.5 | NC_005336.1 | + | 14700 | 0.66 | 0.821163 |
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Target: 5'- -gGCaGCggCUCGA-GGUGCCGcGUGGc -3' miRNA: 3'- agCGaCGa-GAGCUaCCACGGC-CACUa -5' |
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| 25046 | 5' | -57.5 | NC_005336.1 | + | 48721 | 0.66 | 0.789094 |
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Target: 5'- cUCGCUGCUCUCGGacgcgcucgcgGCCGGcaagGAg -3' miRNA: 3'- -AGCGACGAGAGCUacca-------CGGCCa---CUa -5' |
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| 25046 | 5' | -57.5 | NC_005336.1 | + | 130955 | 0.68 | 0.707811 |
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Target: 5'- gUC-CUGCcCUCGGUGa-GCCGGUGAa -3' miRNA: 3'- -AGcGACGaGAGCUACcaCGGCCACUa -5' |
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| 25046 | 5' | -57.5 | NC_005336.1 | + | 103597 | 0.68 | 0.687576 |
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Target: 5'- gCGCUGCggccggCggcggCGAUGGUGcCCGGcGAg -3' miRNA: 3'- aGCGACGa-----Ga----GCUACCAC-GGCCaCUa -5' |
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| 25046 | 5' | -57.5 | NC_005336.1 | + | 93858 | 0.68 | 0.677384 |
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Target: 5'- -gGCUGCcggUCGAccUGGUGCCGGUa-- -3' miRNA: 3'- agCGACGag-AGCU--ACCACGGCCAcua -5' |
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| 25046 | 5' | -57.5 | NC_005336.1 | + | 51480 | 0.69 | 0.667155 |
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Target: 5'- gUCGCgccGCagCU-GGUGGUGCUGGUGGg -3' miRNA: 3'- -AGCGa--CGa-GAgCUACCACGGCCACUa -5' |
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| 25046 | 5' | -57.5 | NC_005336.1 | + | 111983 | 0.7 | 0.605472 |
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Target: 5'- aCGUUGUuuuUCUCGAUGGcGgCGGUGGc -3' miRNA: 3'- aGCGACG---AGAGCUACCaCgGCCACUa -5' |
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| 25046 | 5' | -57.5 | NC_005336.1 | + | 50732 | 0.75 | 0.316063 |
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Target: 5'- cUCGCUGCUCUCGcucuucaacGUGGUGCCGcGcaccaUGAc -3' miRNA: 3'- -AGCGACGAGAGC---------UACCACGGC-C-----ACUa -5' |
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| 25046 | 5' | -57.5 | NC_005336.1 | + | 128549 | 1.05 | 0.002997 |
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Target: 5'- cUCGCUGCUCUCGAUGGUGCCGGUGAUc -3' miRNA: 3'- -AGCGACGAGAGCUACCACGGCCACUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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