Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 25057 | 3' | -57.1 | NC_005336.1 | + | 75229 | 0.71 | 0.566581 |
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Target: 5'- -cUGCGUgaacugCCGGGacuugUCGCGGAUGAg -3' miRNA: 3'- aaGCGCAaa----GGCCCg----AGCGCCUACUg -5' |
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| 25057 | 3' | -57.1 | NC_005336.1 | + | 121515 | 0.71 | 0.566581 |
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Target: 5'- -aCGCGgugCCGGGCcugCGCGGGUacgcGACc -3' miRNA: 3'- aaGCGCaaaGGCCCGa--GCGCCUA----CUG- -5' |
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| 25057 | 3' | -57.1 | NC_005336.1 | + | 52018 | 0.71 | 0.546418 |
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Target: 5'- -gCGCGagUCCGGGCUCcGCGcGAUcGCg -3' miRNA: 3'- aaGCGCaaAGGCCCGAG-CGC-CUAcUG- -5' |
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| 25057 | 3' | -57.1 | NC_005336.1 | + | 73166 | 0.72 | 0.487512 |
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Target: 5'- --gGCGUgUCCGGGUUCagGCGGAaGGCg -3' miRNA: 3'- aagCGCAaAGGCCCGAG--CGCCUaCUG- -5' |
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| 25057 | 3' | -57.1 | NC_005336.1 | + | 101321 | 0.72 | 0.487512 |
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Target: 5'- -cCGCGag-CgCGGGCUCGCGGAccucgcgGACa -3' miRNA: 3'- aaGCGCaaaG-GCCCGAGCGCCUa------CUG- -5' |
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| 25057 | 3' | -57.1 | NC_005336.1 | + | 100792 | 0.76 | 0.296294 |
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Target: 5'- gUCGCGcg-CCgGGGCUCGCGGAcgcgcUGGCc -3' miRNA: 3'- aAGCGCaaaGG-CCCGAGCGCCU-----ACUG- -5' |
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| 25057 | 3' | -57.1 | NC_005336.1 | + | 19817 | 0.76 | 0.296294 |
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Target: 5'- aUCGCGUgUuuGGGCuuUCGCGGAaGGCg -3' miRNA: 3'- aAGCGCAaAggCCCG--AGCGCCUaCUG- -5' |
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| 25057 | 3' | -57.1 | NC_005336.1 | + | 121237 | 0.96 | 0.015649 |
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Target: 5'- gUUCGCGUUcUCGGGCUCGCGGAUGACg -3' miRNA: 3'- -AAGCGCAAaGGCCCGAGCGCCUACUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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