Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2509 | 3' | -55.3 | NC_001454.1 | + | 24937 | 0.66 | 0.543751 |
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Target: 5'- ----cCCGUCGUgCGCgCUUAGGCuGCu -3' miRNA: 3'- uuuuaGGCAGCA-GCGgGAGUCCG-UGc -5' |
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| 2509 | 3' | -55.3 | NC_001454.1 | + | 13779 | 0.66 | 0.543751 |
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Target: 5'- ---uUCCGaauaGUUGCCCUCuGGCAa- -3' miRNA: 3'- uuuuAGGCag--CAGCGGGAGuCCGUgc -5' |
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| 2509 | 3' | -55.3 | NC_001454.1 | + | 7755 | 0.66 | 0.532655 |
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Target: 5'- ----aCCcUCGUCGCCCUCuucuGCACc -3' miRNA: 3'- uuuuaGGcAGCAGCGGGAGuc--CGUGc -5' |
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| 2509 | 3' | -55.3 | NC_001454.1 | + | 16316 | 0.7 | 0.351597 |
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Target: 5'- ----gCCGUCGUCGCUcuguccgccgccgCUCAaGGCGCa -3' miRNA: 3'- uuuuaGGCAGCAGCGG-------------GAGU-CCGUGc -5' |
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| 2509 | 3' | -55.3 | NC_001454.1 | + | 15166 | 0.72 | 0.251784 |
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Target: 5'- ----cCCGUCGUcaggcCGCCCgUCAGGcCGCGg -3' miRNA: 3'- uuuuaGGCAGCA-----GCGGG-AGUCC-GUGC- -5' |
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| 2509 | 3' | -55.3 | NC_001454.1 | + | 12696 | 1.06 | 0.000838 |
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Target: 5'- aAAAAUCCGUCGUCGCCCUCAGGCACGu -3' miRNA: 3'- -UUUUAGGCAGCAGCGGGAGUCCGUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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