Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2509 | 5' | -52.3 | NC_001454.1 | + | 27957 | 0.67 | 0.712458 |
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Target: 5'- aCUCG-GUG--GGCCGGuggaGGUGGCAg -3' miRNA: 3'- -GGGCaCAUuuUUGGCCug--CCACCGUa -5' |
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| 2509 | 5' | -52.3 | NC_001454.1 | + | 9240 | 0.67 | 0.701117 |
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Target: 5'- gUCGcaagGUAA--GCUaaGGACGGUGGCAUg -3' miRNA: 3'- gGGCa---CAUUuuUGG--CCUGCCACCGUA- -5' |
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| 2509 | 5' | -52.3 | NC_001454.1 | + | 21758 | 0.68 | 0.64471 |
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Target: 5'- aCCCGagucgagccaaggugGUAcAAAACCGGugGG-GGCGc -3' miRNA: 3'- -GGGCa--------------CAU-UUUUGGCCugCCaCCGUa -5' |
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| 2509 | 5' | -52.3 | NC_001454.1 | + | 16315 | 0.68 | 0.620357 |
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Target: 5'- uUCCGcgGUA---ACCGGGCGuUGGCGUg -3' miRNA: 3'- -GGGCa-CAUuuuUGGCCUGCcACCGUA- -5' |
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| 2509 | 5' | -52.3 | NC_001454.1 | + | 10325 | 0.69 | 0.54912 |
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Target: 5'- gCCGUugcucucuccGGAGCCGG-CGGUGGCAa -3' miRNA: 3'- gGGCAcau-------UUUUGGCCuGCCACCGUa -5' |
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| 2509 | 5' | -52.3 | NC_001454.1 | + | 12661 | 1.07 | 0.001509 |
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Target: 5'- gCCCGUGUAAAAACCGGACGGUGGCAUc -3' miRNA: 3'- -GGGCACAUUUUUGGCCUGCCACCGUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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