Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2510 | 5' | -64.5 | NC_001454.1 | + | 14714 | 0.66 | 0.210953 |
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Target: 5'- gGGGCaGGUCcGACGGCGGGCgucagUGAUg -3' miRNA: 3'- gCCCG-CCGGaCUGCUGCCCGg----GCUGg -5' |
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| 2510 | 5' | -64.5 | NC_001454.1 | + | 11487 | 0.66 | 0.205643 |
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Target: 5'- uCGGGCGugacacCCUcaGACGACuGGGCggCGGCCa -3' miRNA: 3'- -GCCCGCc-----GGA--CUGCUG-CCCGg-GCUGG- -5' |
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| 2510 | 5' | -64.5 | NC_001454.1 | + | 11287 | 0.66 | 0.204595 |
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Target: 5'- gGGGCGGaCCUGcaauggcagcccaGCGccCGGGCCCuggaggcGGCUg -3' miRNA: 3'- gCCCGCC-GGAC-------------UGCu-GCCCGGG-------CUGG- -5' |
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| 2510 | 5' | -64.5 | NC_001454.1 | + | 12623 | 0.67 | 0.200448 |
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Target: 5'- aCGGGCGaGUUUGACGugccugaGGGCgaCGACg -3' miRNA: 3'- -GCCCGC-CGGACUGCug-----CCCGg-GCUGg -5' |
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| 2510 | 5' | -64.5 | NC_001454.1 | + | 17483 | 0.68 | 0.17163 |
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Target: 5'- aGGGCGGCg-GGCGGgGGuaGCCaCGGCa -3' miRNA: 3'- gCCCGCCGgaCUGCUgCC--CGG-GCUGg -5' |
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| 2510 | 5' | -64.5 | NC_001454.1 | + | 15843 | 0.68 | 0.150501 |
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Target: 5'- gCGGGCuucaGGCUUGGCGugGGGUugcuUUGGuCCa -3' miRNA: 3'- -GCCCG----CCGGACUGCugCCCG----GGCU-GG- -5' |
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| 2510 | 5' | -64.5 | NC_001454.1 | + | 19111 | 0.69 | 0.13533 |
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Target: 5'- aGGGCG--CUGGgGACGGcaaCCCGACCg -3' miRNA: 3'- gCCCGCcgGACUgCUGCCc--GGGCUGG- -5' |
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| 2510 | 5' | -64.5 | NC_001454.1 | + | 8933 | 0.7 | 0.118347 |
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Target: 5'- aCGGGuuGUCgucGACGACGGcGCCgGACg -3' miRNA: 3'- -GCCCgcCGGa--CUGCUGCC-CGGgCUGg -5' |
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| 2510 | 5' | -64.5 | NC_001454.1 | + | 15219 | 1.11 | 6.6e-05 |
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Target: 5'- aCGGGCGGCCUGACGACGGGCCCGACCu -3' miRNA: 3'- -GCCCGCCGGACUGCUGCCCGGGCUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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