Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 25100 | 5' | -59.1 | NC_005336.1 | + | 21079 | 0.66 | 0.707811 |
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Target: 5'- cGGCA-CCGGGGUCUuGUucGGUGCCUUg -3' miRNA: 3'- -UCGUaGGUCUCAGAcCA--CCGCGGGAa -5' |
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| 25100 | 5' | -59.1 | NC_005336.1 | + | 17934 | 0.67 | 0.646619 |
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Target: 5'- cGCGUCCAuGGccaUCUGGUGGUccgagcagGCCCg- -3' miRNA: 3'- uCGUAGGUcUC---AGACCACCG--------CGGGaa -5' |
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| 25100 | 5' | -59.1 | NC_005336.1 | + | 40413 | 0.68 | 0.574793 |
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Target: 5'- aGGCGUCC-GAGUCcaUGaUGGCGUCCa- -3' miRNA: 3'- -UCGUAGGuCUCAG--ACcACCGCGGGaa -5' |
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| 25100 | 5' | -59.1 | NC_005336.1 | + | 30709 | 0.69 | 0.514713 |
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Target: 5'- uGGC--CCAGAGUCUGGUccaGCGCCUc- -3' miRNA: 3'- -UCGuaGGUCUCAGACCAc--CGCGGGaa -5' |
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| 25100 | 5' | -59.1 | NC_005336.1 | + | 108973 | 0.69 | 0.514713 |
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Target: 5'- uGCAaCCAGccgCUGGUGcGCGCCCg- -3' miRNA: 3'- uCGUaGGUCucaGACCAC-CGCGGGaa -5' |
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| 25100 | 5' | -59.1 | NC_005336.1 | + | 87857 | 0.69 | 0.504934 |
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Target: 5'- cGCGUCCguccgcgauGGAGUCguaGUGGUGCCCc- -3' miRNA: 3'- uCGUAGG---------UCUCAGac-CACCGCGGGaa -5' |
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| 25100 | 5' | -59.1 | NC_005336.1 | + | 90034 | 0.69 | 0.504934 |
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Target: 5'- gAGCAccUCCAGc--CUGGUGGUGCUCUc -3' miRNA: 3'- -UCGU--AGGUCucaGACCACCGCGGGAa -5' |
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| 25100 | 5' | -59.1 | NC_005336.1 | + | 78155 | 0.69 | 0.504934 |
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Target: 5'- cGCGUCCGGcgcgcgcgcGGgcgUGGUGGUGCCCg- -3' miRNA: 3'- uCGUAGGUC---------UCag-ACCACCGCGGGaa -5' |
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| 25100 | 5' | -59.1 | NC_005336.1 | + | 102437 | 1.05 | 0.002084 |
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Target: 5'- gAGCAUCCAGAGUCUGGUGGCGCCCUUc -3' miRNA: 3'- -UCGUAGGUCUCAGACCACCGCGGGAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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