Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2513 | 3' | -50.9 | NC_001454.1 | + | 11582 | 0.66 | 0.830266 |
Target: 5'- gGUGGUuagggccaggauGCGA--UCCAUggcCgCCGCCCa -3' miRNA: 3'- -CACCG------------UGCUuaAGGUAaauG-GGCGGG- -5' |
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2513 | 3' | -50.9 | NC_001454.1 | + | 16160 | 0.67 | 0.746795 |
Target: 5'- cGUGGUACGAAUUCgug--GCCCcuaccagccGUCCa -3' miRNA: 3'- -CACCGUGCUUAAGguaaaUGGG---------CGGG- -5' |
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2513 | 3' | -50.9 | NC_001454.1 | + | 17386 | 0.68 | 0.678248 |
Target: 5'- uUGGCACcg---CCGUUU-CUCGCCCc -3' miRNA: 3'- cACCGUGcuuaaGGUAAAuGGGCGGG- -5' |
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2513 | 3' | -50.9 | NC_001454.1 | + | 15164 | 0.74 | 0.361745 |
Target: 5'- aUGGCcCGAcgUCCuauccuuugUGCCCGCCUu -3' miRNA: 3'- cACCGuGCUuaAGGuaa------AUGGGCGGG- -5' |
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2513 | 3' | -50.9 | NC_001454.1 | + | 25573 | 1.14 | 0.00073 |
Target: 5'- aGUGGCACGAAUUCCAUUUACCCGCCCa -3' miRNA: 3'- -CACCGUGCUUAAGGUAAAUGGGCGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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