Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2514 | 3' | -52.6 | NC_001454.1 | + | 16375 | 0.66 | 0.692452 |
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Target: 5'- aGCGGCGgCGGacagagcgacgacgGCGGGUGgagcgagUGCGGCUg -3' miRNA: 3'- -UGCCGUgGCUa-------------CGUCUACa------AUGUCGG- -5' |
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| 2514 | 3' | -52.6 | NC_001454.1 | + | 9620 | 0.66 | 0.686698 |
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Target: 5'- aGCGGUgguaggaACCGGUGUugauGGUGUacgUGCAGUUg -3' miRNA: 3'- -UGCCG-------UGGCUACGu---CUACA---AUGUCGG- -5' |
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| 2514 | 3' | -52.6 | NC_001454.1 | + | 24657 | 0.67 | 0.676305 |
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Target: 5'- gGCGGaggaGCaGAUGgAGAgcUGggACAGCCu -3' miRNA: 3'- -UGCCg---UGgCUACgUCU--ACaaUGUCGG- -5' |
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| 2514 | 3' | -52.6 | NC_001454.1 | + | 11598 | 0.67 | 0.676305 |
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Target: 5'- -aGGCuuCGGgauUGCGGGUgGUUAgGGCCa -3' miRNA: 3'- ugCCGugGCU---ACGUCUA-CAAUgUCGG- -5' |
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| 2514 | 3' | -52.6 | NC_001454.1 | + | 6239 | 0.67 | 0.653088 |
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Target: 5'- cACGGCAUgGGauggguaagUGCAGAggcgUACAuGCCa -3' miRNA: 3'- -UGCCGUGgCU---------ACGUCUaca-AUGU-CGG- -5' |
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| 2514 | 3' | -52.6 | NC_001454.1 | + | 27955 | 0.68 | 0.618129 |
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Target: 5'- uCGGUggGCCGGUGgAGGUGgcaguUGGCCa -3' miRNA: 3'- uGCCG--UGGCUACgUCUACaau--GUCGG- -5' |
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| 2514 | 3' | -52.6 | NC_001454.1 | + | 27770 | 0.7 | 0.49305 |
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Target: 5'- gUGGCAauGcUGCAGAUGgcagUGCAGCa -3' miRNA: 3'- uGCCGUggCuACGUCUACa---AUGUCGg -5' |
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| 2514 | 3' | -52.6 | NC_001454.1 | + | 10634 | 0.73 | 0.327257 |
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Target: 5'- cCGGCACgGgcGCAcGUGUcgGCGGCCg -3' miRNA: 3'- uGCCGUGgCuaCGUcUACAa-UGUCGG- -5' |
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| 2514 | 3' | -52.6 | NC_001454.1 | + | 26179 | 1.13 | 0.000527 |
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Target: 5'- uACGGCACCGAUGCAGAUGUUACAGCCg -3' miRNA: 3'- -UGCCGUGGCUACGUCUACAAUGUCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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