Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 25141 | 5' | -60.8 | NC_005336.1 | + | 87233 | 0.66 | 0.670656 |
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Target: 5'- cAGGGGucguGCuuGcGCCCGUCGAUGa-- -3' miRNA: 3'- uUCCCCu---CGucC-CGGGCAGCUACaga -5' |
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| 25141 | 5' | -60.8 | NC_005336.1 | + | 10535 | 0.66 | 0.650549 |
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Target: 5'- -cGGGcGGCGGGGCUagcgCGUCGGUG-Cg -3' miRNA: 3'- uuCCCcUCGUCCCGG----GCAGCUACaGa -5' |
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| 25141 | 5' | -60.8 | NC_005336.1 | + | 29545 | 0.67 | 0.600167 |
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Target: 5'- -cGGGuccaGGuCAGGGUCCGUCG-UGUCg -3' miRNA: 3'- uuCCCc---UC-GUCCCGGGCAGCuACAGa -5' |
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| 25141 | 5' | -60.8 | NC_005336.1 | + | 23620 | 0.67 | 0.59013 |
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Target: 5'- cGGGGuGGGGUcggAGGGCCUGUacAUGUCUu -3' miRNA: 3'- -UUCC-CCUCG---UCCCGGGCAgcUACAGA- -5' |
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| 25141 | 5' | -60.8 | NC_005336.1 | + | 19584 | 0.68 | 0.521053 |
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Target: 5'- uGGGGGGAG-GGGGCCCGccCGAauUCUc -3' miRNA: 3'- -UUCCCCUCgUCCCGGGCa-GCUacAGA- -5' |
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| 25141 | 5' | -60.8 | NC_005336.1 | + | 41291 | 0.7 | 0.428584 |
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Target: 5'- gAAGGGGGGCucuaAGGGaggcgUgGUCGAUGUCg -3' miRNA: 3'- -UUCCCCUCG----UCCCg----GgCAGCUACAGa -5' |
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| 25141 | 5' | -60.8 | NC_005336.1 | + | 76741 | 1.05 | 0.001499 |
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Target: 5'- cAAGGGGAGCAGGGCCCGUCGAUGUCUa -3' miRNA: 3'- -UUCCCCUCGUCCCGGGCAGCUACAGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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