Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2515 | 3' | -59.6 | NC_001454.1 | + | 11422 | 0.67 | 0.297184 |
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Target: 5'- --cGGCGGUGGCgGacGGGUCGCAgcaggugaaUCCc -3' miRNA: 3'- gacCCGCCGCCGgUa-CCUAGCGU---------AGG- -5' |
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| 2515 | 3' | -59.6 | NC_001454.1 | + | 11584 | 0.68 | 0.268527 |
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Target: 5'- -cGGGUGGUuagGGCCA-GGAU-GCgAUCCa -3' miRNA: 3'- gaCCCGCCG---CCGGUaCCUAgCG-UAGG- -5' |
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| 2515 | 3' | -59.6 | NC_001454.1 | + | 10335 | 0.7 | 0.182568 |
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Target: 5'- -gGGaGCGGCGGCUGUguuggaggcgccgucGGA-CGCAUCUg -3' miRNA: 3'- gaCC-CGCCGCCGGUA---------------CCUaGCGUAGG- -5' |
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| 2515 | 3' | -59.6 | NC_001454.1 | + | 16066 | 0.72 | 0.133319 |
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Target: 5'- aCUGcGGUGGaGGCCAUGGAaaCGCAgCCu -3' miRNA: 3'- -GAC-CCGCCgCCGGUACCUa-GCGUaGG- -5' |
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| 2515 | 3' | -59.6 | NC_001454.1 | + | 25658 | 0.75 | 0.089647 |
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Target: 5'- cCUGGGCGGCaGGCCAagaGGGUgGguUCa -3' miRNA: 3'- -GACCCGCCG-CCGGUa--CCUAgCguAGg -5' |
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| 2515 | 3' | -59.6 | NC_001454.1 | + | 11509 | 1.11 | 0.000134 |
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Target: 5'- aCUGGGCGGCGGCCAUGGAUCGCAUCCu -3' miRNA: 3'- -GACCCGCCGCCGGUACCUAGCGUAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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