Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 25150 | 3' | -58.9 | NC_005336.1 | + | 68083 | 0.66 | 0.774487 |
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Target: 5'- -cGGGCACGGacucgUCgUGCCCGGcgaacagCUUGAUc -3' miRNA: 3'- caCUCGUGCC-----AG-ACGGGCCa------GAGCUA- -5' |
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| 25150 | 3' | -58.9 | NC_005336.1 | + | 63410 | 0.67 | 0.676776 |
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Target: 5'- -cGAGCAgcCGcGUCUGCgUGGUCaUCGAc -3' miRNA: 3'- caCUCGU--GC-CAGACGgGCCAG-AGCUa -5' |
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| 25150 | 3' | -58.9 | NC_005336.1 | + | 36440 | 0.67 | 0.673745 |
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Target: 5'- -cGAGCGCGuUCUcGacggugaccgcgaaCCCGGUCUCGAa -3' miRNA: 3'- caCUCGUGCcAGA-C--------------GGGCCAGAGCUa -5' |
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| 25150 | 3' | -58.9 | NC_005336.1 | + | 98172 | 0.68 | 0.626001 |
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Target: 5'- cGUGGGCGCGGUCcugggGCUCGcGcaccugCUCGAg -3' miRNA: 3'- -CACUCGUGCCAGa----CGGGC-Ca-----GAGCUa -5' |
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| 25150 | 3' | -58.9 | NC_005336.1 | + | 93507 | 0.68 | 0.626001 |
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Target: 5'- --aGGCGCGGcugCUGCCCGGcagCUCGc- -3' miRNA: 3'- cacUCGUGCCa--GACGGGCCa--GAGCua -5' |
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| 25150 | 3' | -58.9 | NC_005336.1 | + | 110729 | 0.69 | 0.585411 |
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Target: 5'- --cGGCGCGGUC-GCCCuugucgcccuuGGUCUCGGc -3' miRNA: 3'- cacUCGUGCCAGaCGGG-----------CCAGAGCUa -5' |
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| 25150 | 3' | -58.9 | NC_005336.1 | + | 74974 | 0.69 | 0.555293 |
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Target: 5'- -cGAGgGCGGUCUGCgagcgccaggUCGGcCUCGGUg -3' miRNA: 3'- caCUCgUGCCAGACG----------GGCCaGAGCUA- -5' |
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| 25150 | 3' | -58.9 | NC_005336.1 | + | 62782 | 0.74 | 0.297669 |
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Target: 5'- aUGAGCGCGGaUCgcgcgGCCgCGGUCUCGc- -3' miRNA: 3'- cACUCGUGCC-AGa----CGG-GCCAGAGCua -5' |
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| 25150 | 3' | -58.9 | NC_005336.1 | + | 68899 | 1.06 | 0.002055 |
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Target: 5'- cGUGAGCACGGUCUGCCCGGUCUCGAUg -3' miRNA: 3'- -CACUCGUGCCAGACGGGCCAGAGCUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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