Results 1 - 20 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2516 | 3' | -59.3 | NC_001454.1 | + | 14659 | 0.66 | 0.376837 |
Target: 5'- cUGCugCGCaGCGGCaGGguuCCGUGAucugugAgGGCg -3' miRNA: 3'- -ACGugGCG-CGCCG-CC---GGUACU------UgUCG- -5' |
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2516 | 3' | -59.3 | NC_001454.1 | + | 16113 | 0.66 | 0.367973 |
Target: 5'- aGC-CCGUGCGGUugcGGCCAcgcgagaGAugGGUu -3' miRNA: 3'- aCGuGGCGCGCCG---CCGGUa------CUugUCG- -5' |
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2516 | 3' | -59.3 | NC_001454.1 | + | 11409 | 0.67 | 0.325835 |
Target: 5'- aUGCugCGUucgacgGCGGUGGCgGacgGGucGCAGCa -3' miRNA: 3'- -ACGugGCG------CGCCGCCGgUa--CU--UGUCG- -5' |
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2516 | 3' | -59.3 | NC_001454.1 | + | 6559 | 0.67 | 0.310012 |
Target: 5'- uUGCACCaGgGCGGCGGuaacaagcacauCCAgGGcACAGUa -3' miRNA: 3'- -ACGUGG-CgCGCCGCC------------GGUaCU-UGUCG- -5' |
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2516 | 3' | -59.3 | NC_001454.1 | + | 20750 | 0.67 | 0.310012 |
Target: 5'- aGgACCGUuuuCGGCGGCCu---GCGGCc -3' miRNA: 3'- aCgUGGCGc--GCCGCCGGuacuUGUCG- -5' |
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2516 | 3' | -59.3 | NC_001454.1 | + | 9900 | 0.67 | 0.310012 |
Target: 5'- gGUgauGCCG-GCGGCGGUaGUG-GCGGCg -3' miRNA: 3'- aCG---UGGCgCGCCGCCGgUACuUGUCG- -5' |
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2516 | 3' | -59.3 | NC_001454.1 | + | 11235 | 0.67 | 0.287387 |
Target: 5'- aGC-UCGCGaCGGCGGCUGgcgcucACAGCu -3' miRNA: 3'- aCGuGGCGC-GCCGCCGGUacu---UGUCG- -5' |
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2516 | 3' | -59.3 | NC_001454.1 | + | 16385 | 0.67 | 0.287387 |
Target: 5'- gUGCGCCuugaGCGGCGGC---GGACAGa -3' miRNA: 3'- -ACGUGGcg--CGCCGCCGguaCUUGUCg -5' |
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2516 | 3' | -59.3 | NC_001454.1 | + | 10733 | 0.68 | 0.273038 |
Target: 5'- cGCACCcugguggcGCGCgaggaGGUGGCCAUuGGGCugauGCa -3' miRNA: 3'- aCGUGG--------CGCG-----CCGCCGGUA-CUUGu---CG- -5' |
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2516 | 3' | -59.3 | NC_001454.1 | + | 32067 | 0.68 | 0.25927 |
Target: 5'- gUGCugCGCucgucggacGCGGCGGCUGcUGGccaagGCGGUg -3' miRNA: 3'- -ACGugGCG---------CGCCGCCGGU-ACU-----UGUCG- -5' |
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2516 | 3' | -59.3 | NC_001454.1 | + | 16600 | 0.68 | 0.2526 |
Target: 5'- cUGCGCaaagCGCGuCGGCGGCCAcugcccgccaUGccucuccuACGGCg -3' miRNA: 3'- -ACGUG----GCGC-GCCGCCGGU----------ACu-------UGUCG- -5' |
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2516 | 3' | -59.3 | NC_001454.1 | + | 15179 | 0.69 | 0.233439 |
Target: 5'- cGC-CCGUcaggccGCGGCGGCUAUugcCAGCa -3' miRNA: 3'- aCGuGGCG------CGCCGCCGGUAcuuGUCG- -5' |
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2516 | 3' | -59.3 | NC_001454.1 | + | 10642 | 0.7 | 0.198782 |
Target: 5'- gGCGCaCGUGuCGGCGGCCGauuUGGuaACcGCg -3' miRNA: 3'- aCGUG-GCGC-GCCGCCGGU---ACU--UGuCG- -5' |
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2516 | 3' | -59.3 | NC_001454.1 | + | 11890 | 0.7 | 0.188266 |
Target: 5'- aUGUGCCGCGCGGgcaggaGGaCUAUGu-CAGCu -3' miRNA: 3'- -ACGUGGCGCGCCg-----CC-GGUACuuGUCG- -5' |
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2516 | 3' | -59.3 | NC_001454.1 | + | 17492 | 0.7 | 0.183193 |
Target: 5'- aGCcCCGCGagGGCGGCgGgcggGggUAGCc -3' miRNA: 3'- aCGuGGCGCg-CCGCCGgUa---CuuGUCG- -5' |
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2516 | 3' | -59.3 | NC_001454.1 | + | 141 | 0.71 | 0.164095 |
Target: 5'- gGCGCgGUGCGGCGGaaGUGA-CGGa -3' miRNA: 3'- aCGUGgCGCGCCGCCggUACUuGUCg -5' |
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2516 | 3' | -59.3 | NC_001454.1 | + | 10976 | 0.71 | 0.159609 |
Target: 5'- aGCugGCCGacaagGUGGCGGCCAUuaauuACAGCa -3' miRNA: 3'- aCG--UGGCg----CGCCGCCGGUAcu---UGUCG- -5' |
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2516 | 3' | -59.3 | NC_001454.1 | + | 34074 | 0.73 | 0.110631 |
Target: 5'- gGCGCgGUGCGGCGGaaGUGAcgauaugugaaACAGUa -3' miRNA: 3'- aCGUGgCGCGCCGCCggUACU-----------UGUCG- -5' |
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2516 | 3' | -59.3 | NC_001454.1 | + | 16662 | 0.74 | 0.104474 |
Target: 5'- uUGCGCCuaugGCGGCGGCgAUGAugGGa -3' miRNA: 3'- -ACGUGGcg--CGCCGCCGgUACUugUCg -5' |
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2516 | 3' | -59.3 | NC_001454.1 | + | 15131 | 0.74 | 0.101518 |
Target: 5'- aGUACCGCccgaGCGGCaGUCAUG-GCGGCa -3' miRNA: 3'- aCGUGGCG----CGCCGcCGGUACuUGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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