Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2517 | 5' | -56.7 | NC_001454.1 | + | 707 | 0.67 | 0.423797 |
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Target: 5'- cGCuGCAGagAGCGGuuCGGGUGaUUCu -3' miRNA: 3'- uCGuCGUCg-UCGCCu-GCCCACaAAGc -5' |
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| 2517 | 5' | -56.7 | NC_001454.1 | + | 17487 | 0.67 | 0.423797 |
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Target: 5'- cGCGagGGCGGCGGGCGGGgGUa--- -3' miRNA: 3'- uCGUcgUCGUCGCCUGCCCaCAaagc -5' |
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| 2517 | 5' | -56.7 | NC_001454.1 | + | 15560 | 0.68 | 0.36745 |
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Target: 5'- cGGCGGguGCAGUGG-CGGGgacGUa--- -3' miRNA: 3'- -UCGUCguCGUCGCCuGCCCa--CAaagc -5' |
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| 2517 | 5' | -56.7 | NC_001454.1 | + | 24549 | 0.68 | 0.36745 |
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Target: 5'- aAGCGGCGGCGGagaGuGACGGG-GUa--- -3' miRNA: 3'- -UCGUCGUCGUCg--C-CUGCCCaCAaagc -5' |
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| 2517 | 5' | -56.7 | NC_001454.1 | + | 8118 | 0.7 | 0.284811 |
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Target: 5'- gGGCguccggaGGCAGUGGUGGAUGGGgg-UUCGc -3' miRNA: 3'- -UCG-------UCGUCGUCGCCUGCCCacaAAGC- -5' |
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| 2517 | 5' | -56.7 | NC_001454.1 | + | 24839 | 0.7 | 0.278205 |
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Target: 5'- uGCGGUGGCAG-GGGCGGGauaguUUUCGg -3' miRNA: 3'- uCGUCGUCGUCgCCUGCCCac---AAAGC- -5' |
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| 2517 | 5' | -56.7 | NC_001454.1 | + | 16382 | 0.71 | 0.235353 |
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Target: 5'- cGCcuugAGCGGCGGCGGacagagcgacgacgGCGGGUGgagCGa -3' miRNA: 3'- uCG----UCGUCGUCGCC--------------UGCCCACaaaGC- -5' |
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| 2517 | 5' | -56.7 | NC_001454.1 | + | 11419 | 0.72 | 0.201487 |
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Target: 5'- cGaCGGCGGUGGCGGACGGGUc----- -3' miRNA: 3'- uC-GUCGUCGUCGCCUGCCCAcaaagc -5' |
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| 2517 | 5' | -56.7 | NC_001454.1 | + | 9911 | 0.74 | 0.135828 |
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Target: 5'- cGGCGGUAGUGGCGGcGCGGGcGUaUUCGc -3' miRNA: 3'- -UCGUCGUCGUCGCC-UGCCCaCA-AAGC- -5' |
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| 2517 | 5' | -56.7 | NC_001454.1 | + | 12799 | 1.09 | 0.000342 |
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Target: 5'- cAGCAGCAGCAGCGGACGGGUGUUUCGa -3' miRNA: 3'- -UCGUCGUCGUCGCCUGCCCACAAAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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