Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2518 | 3' | -56.9 | NC_001454.1 | + | 14772 | 0.66 | 0.473614 |
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Target: 5'- uACCGGCuggggacuuugcuccGCCggCAuGUaCGgcGGCGCCa -3' miRNA: 3'- cUGGCCG---------------UGGaaGU-CA-GCaaCCGCGG- -5' |
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| 2518 | 3' | -56.9 | NC_001454.1 | + | 25837 | 0.67 | 0.429298 |
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Target: 5'- cACCGGUccuagACCUgacggagucuuUCAGcUUGgaGGCGCCg -3' miRNA: 3'- cUGGCCG-----UGGA-----------AGUC-AGCaaCCGCGG- -5' |
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| 2518 | 3' | -56.9 | NC_001454.1 | + | 9007 | 0.67 | 0.429298 |
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Target: 5'- uGACCGGCugCcc--GUCc--GGCGCCg -3' miRNA: 3'- -CUGGCCGugGaaguCAGcaaCCGCGG- -5' |
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| 2518 | 3' | -56.9 | NC_001454.1 | + | 16390 | 0.68 | 0.400522 |
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Target: 5'- gGGCCGuGCGCCUUgAG-CGgcGGCGg- -3' miRNA: 3'- -CUGGC-CGUGGAAgUCaGCaaCCGCgg -5' |
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| 2518 | 3' | -56.9 | NC_001454.1 | + | 10334 | 0.68 | 0.382033 |
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Target: 5'- gGAgCGGCGgCUgu-GUUGgaGGCGCCg -3' miRNA: 3'- -CUgGCCGUgGAaguCAGCaaCCGCGG- -5' |
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| 2518 | 3' | -56.9 | NC_001454.1 | + | 16799 | 0.7 | 0.283521 |
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Target: 5'- aGAUCGGCACCagcCAGcucaaCGggGGCGCUu -3' miRNA: 3'- -CUGGCCGUGGaa-GUCa----GCaaCCGCGG- -5' |
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| 2518 | 3' | -56.9 | NC_001454.1 | + | 8919 | 0.72 | 0.203986 |
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Target: 5'- uGACCGGCGCUgggacggguuGUCGUcgacgacGGCGCCg -3' miRNA: 3'- -CUGGCCGUGGaagu------CAGCAa------CCGCGG- -5' |
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| 2518 | 3' | -56.9 | NC_001454.1 | + | 12986 | 1.12 | 0.000229 |
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Target: 5'- uGACCGGCACCUUCAGUCGUUGGCGCCg -3' miRNA: 3'- -CUGGCCGUGGAAGUCAGCAACCGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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