Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2519 | 3' | -51.3 | NC_001454.1 | + | 15234 | 0.66 | 0.811231 |
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Target: 5'- aGCCGCCG-CGGccugacgggcggccUGAcGACGggCCCGa -3' miRNA: 3'- -UGGUGGCaGUC--------------ACUuUUGCaaGGGCg -5' |
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| 2519 | 3' | -51.3 | NC_001454.1 | + | 1165 | 0.66 | 0.810236 |
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Target: 5'- cGCCGCCacauggaauuGUGAGAACcaUCCCGUg -3' miRNA: 3'- -UGGUGGcagu------CACUUUUGcaAGGGCG- -5' |
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| 2519 | 3' | -51.3 | NC_001454.1 | + | 14611 | 0.66 | 0.79813 |
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Target: 5'- uGCCGCUGcgcagcaguaUCAGUGGAGuucagcGCGUgaccaucacugacgCCCGCc -3' miRNA: 3'- -UGGUGGC----------AGUCACUUU------UGCAa-------------GGGCG- -5' |
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| 2519 | 3' | -51.3 | NC_001454.1 | + | 22399 | 0.67 | 0.783657 |
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Target: 5'- uGCCuCCGUCucccGAAGugGUUCCagacaGCg -3' miRNA: 3'- -UGGuGGCAGuca-CUUUugCAAGGg----CG- -5' |
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| 2519 | 3' | -51.3 | NC_001454.1 | + | 12732 | 0.68 | 0.695459 |
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Target: 5'- uGCCGCUGUCguuAGUGGAGGCagcUUCUCGa -3' miRNA: 3'- -UGGUGGCAG---UCACUUUUGc--AAGGGCg -5' |
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| 2519 | 3' | -51.3 | NC_001454.1 | + | 14564 | 1.11 | 0.001137 |
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Target: 5'- uACCACCGUCAGUGAAAACGUUCCCGCc -3' miRNA: 3'- -UGGUGGCAGUCACUUUUGCAAGGGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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