Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2519 | 5' | -59.9 | NC_001454.1 | + | 13376 | 0.66 | 0.322765 |
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Target: 5'- aCGUGGAGCgCCUGCagCGUUGC-CGGCAa -3' miRNA: 3'- aGUGCCUUG-GGACG--GCGACGcGUCGU- -5' |
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| 2519 | 5' | -59.9 | NC_001454.1 | + | 16809 | 0.66 | 0.322765 |
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Target: 5'- cCGC-GAGCCgUGCCGCggaGC-CAGCGa -3' miRNA: 3'- aGUGcCUUGGgACGGCGa--CGcGUCGU- -5' |
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| 2519 | 5' | -59.9 | NC_001454.1 | + | 1887 | 0.66 | 0.314825 |
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Target: 5'- gCACuGGAuccaaACCgUGCggaCGCUGCGcCGGCAa -3' miRNA: 3'- aGUG-CCU-----UGGgACG---GCGACGC-GUCGU- -5' |
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| 2519 | 5' | -59.9 | NC_001454.1 | + | 12719 | 0.67 | 0.291896 |
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Target: 5'- -gGCGGAGacgagCUGCCGCUGUcguuaguggagGCAGCu -3' miRNA: 3'- agUGCCUUgg---GACGGCGACG-----------CGUCGu -5' |
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| 2519 | 5' | -59.9 | NC_001454.1 | + | 14683 | 0.68 | 0.224912 |
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Target: 5'- gUCACGcgcuGAACUccaCUGauaCUGCUGCGCAGCGg -3' miRNA: 3'- -AGUGC----CUUGG---GAC---GGCGACGCGUCGU- -5' |
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| 2519 | 5' | -59.9 | NC_001454.1 | + | 14599 | 1.09 | 0.000164 |
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Target: 5'- aUCACGGAACCCUGCCGCUGCGCAGCAg -3' miRNA: 3'- -AGUGCCUUGGGACGGCGACGCGUCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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