Results 1 - 20 of 158 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
25256 | 5' | -57.6 | NC_005336.1 | + | 132628 | 0.66 | 0.827913 |
Target: 5'- cCGCGCGCUCguggcaaCUGaCGCggacgugaacuuccgCGGCGAGUa -3' miRNA: 3'- aGCGCGCGAGa------GGC-GUGa--------------GUCGUUCA- -5' |
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25256 | 5' | -57.6 | NC_005336.1 | + | 105328 | 0.66 | 0.827067 |
Target: 5'- cCGCGCgaguGCUCgcugaCCGUGCUCGGUcucGAGg -3' miRNA: 3'- aGCGCG----CGAGa----GGCGUGAGUCG---UUCa -5' |
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25256 | 5' | -57.6 | NC_005336.1 | + | 17692 | 0.66 | 0.827067 |
Target: 5'- aCGCGCGCcuggCUCCGUAUgUAGCc--- -3' miRNA: 3'- aGCGCGCGa---GAGGCGUGaGUCGuuca -5' |
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25256 | 5' | -57.6 | NC_005336.1 | + | 16615 | 0.66 | 0.827067 |
Target: 5'- gCGCGCGCg-UCCGC-CUCcaacacgcGCGAGg -3' miRNA: 3'- aGCGCGCGagAGGCGuGAGu-------CGUUCa -5' |
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25256 | 5' | -57.6 | NC_005336.1 | + | 70413 | 0.66 | 0.827067 |
Target: 5'- gCGCGgGUcCUCCgaGCGC-CGGCAGGa -3' miRNA: 3'- aGCGCgCGaGAGG--CGUGaGUCGUUCa -5' |
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25256 | 5' | -57.6 | NC_005336.1 | + | 134350 | 0.66 | 0.827067 |
Target: 5'- cUGCGCGCUCg-CGCGCgUGGCGAc- -3' miRNA: 3'- aGCGCGCGAGagGCGUGaGUCGUUca -5' |
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25256 | 5' | -57.6 | NC_005336.1 | + | 11690 | 0.66 | 0.827067 |
Target: 5'- gCGCGCaGCUCgUCCGCguccuuguccuuGCUCuGCAcGUc -3' miRNA: 3'- aGCGCG-CGAG-AGGCG------------UGAGuCGUuCA- -5' |
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25256 | 5' | -57.6 | NC_005336.1 | + | 74293 | 0.66 | 0.827067 |
Target: 5'- aCGCGCGUcUUCCGCgacccGCUgCAGCAc-- -3' miRNA: 3'- aGCGCGCGaGAGGCG-----UGA-GUCGUuca -5' |
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25256 | 5' | -57.6 | NC_005336.1 | + | 135949 | 0.66 | 0.818513 |
Target: 5'- aCGCGCGCgg---GCGCUCGGCGGc- -3' miRNA: 3'- aGCGCGCGagaggCGUGAGUCGUUca -5' |
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25256 | 5' | -57.6 | NC_005336.1 | + | 40148 | 0.66 | 0.818513 |
Target: 5'- cUCGCGCGCg---CGCGCgUCcGCGAGg -3' miRNA: 3'- -AGCGCGCGagagGCGUG-AGuCGUUCa -5' |
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25256 | 5' | -57.6 | NC_005336.1 | + | 58502 | 0.66 | 0.818513 |
Target: 5'- gCGCGCGCg---CGCGCUC-GCGGGc -3' miRNA: 3'- aGCGCGCGagagGCGUGAGuCGUUCa -5' |
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25256 | 5' | -57.6 | NC_005336.1 | + | 14990 | 0.66 | 0.818513 |
Target: 5'- aCGCGCGUcaUUCCGUACcgCAGguGGa -3' miRNA: 3'- aGCGCGCGa-GAGGCGUGa-GUCguUCa -5' |
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25256 | 5' | -57.6 | NC_005336.1 | + | 44467 | 0.66 | 0.818513 |
Target: 5'- cUCGCGCGCgcacaugaUCgCCG-ACUgCGGCAAGg -3' miRNA: 3'- -AGCGCGCG--------AGaGGCgUGA-GUCGUUCa -5' |
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25256 | 5' | -57.6 | NC_005336.1 | + | 120459 | 0.66 | 0.818513 |
Target: 5'- cUCGgGCGCUacgacgUCCaacaGCACUucugCAGCAAGUg -3' miRNA: 3'- -AGCgCGCGAg-----AGG----CGUGA----GUCGUUCA- -5' |
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25256 | 5' | -57.6 | NC_005336.1 | + | 135949 | 0.66 | 0.818513 |
Target: 5'- aCGCGCGCgg---GCGCUCGGCGGc- -3' miRNA: 3'- aGCGCGCGagaggCGUGAGUCGUUca -5' |
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25256 | 5' | -57.6 | NC_005336.1 | + | 46395 | 0.66 | 0.818513 |
Target: 5'- aCGCGCGCgC-CCGCGCggGGCGc-- -3' miRNA: 3'- aGCGCGCGaGaGGCGUGagUCGUuca -5' |
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25256 | 5' | -57.6 | NC_005336.1 | + | 73276 | 0.66 | 0.818513 |
Target: 5'- cCGCGCGC---CCGCACccgCAGCucGUg -3' miRNA: 3'- aGCGCGCGagaGGCGUGa--GUCGuuCA- -5' |
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25256 | 5' | -57.6 | NC_005336.1 | + | 39144 | 0.66 | 0.809787 |
Target: 5'- gUGCGCGCUCUUCGCggagAC-CAuCGAGg -3' miRNA: 3'- aGCGCGCGAGAGGCG----UGaGUcGUUCa -5' |
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25256 | 5' | -57.6 | NC_005336.1 | + | 118218 | 0.66 | 0.809787 |
Target: 5'- -aGgGCGCUCaCCGCGCUgaCGGCGGc- -3' miRNA: 3'- agCgCGCGAGaGGCGUGA--GUCGUUca -5' |
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25256 | 5' | -57.6 | NC_005336.1 | + | 31506 | 0.66 | 0.809787 |
Target: 5'- -aGCGCGUa---CGCGCUCAGCAc-- -3' miRNA: 3'- agCGCGCGagagGCGUGAGUCGUuca -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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