Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2528 | 5' | -52 | NC_001454.1 | + | 16150 | 0.66 | 0.728254 |
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Target: 5'- -gGCUacgGCCUCUGGCAGcuCAgGCUgCg -3' miRNA: 3'- uaCGGa--UGGAGACUGUUu-GUgCGAgG- -5' |
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| 2528 | 5' | -52 | NC_001454.1 | + | 22201 | 0.67 | 0.69379 |
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Target: 5'- uUGUUUuCCUCUGACGggggaGGCACaccuuuggGCUCCa -3' miRNA: 3'- uACGGAuGGAGACUGU-----UUGUG--------CGAGG- -5' |
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| 2528 | 5' | -52 | NC_001454.1 | + | 7763 | 0.67 | 0.67045 |
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Target: 5'- -cGCCcuCUUCUGcACcuGCACGCUCUu -3' miRNA: 3'- uaCGGauGGAGAC-UGuuUGUGCGAGG- -5' |
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| 2528 | 5' | -52 | NC_001454.1 | + | 32733 | 0.69 | 0.564804 |
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Target: 5'- -aGCUucgUACUUCUGuuuuaagaaACGAACACGCUgCCa -3' miRNA: 3'- uaCGG---AUGGAGAC---------UGUUUGUGCGA-GG- -5' |
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| 2528 | 5' | -52 | NC_001454.1 | + | 32207 | 0.71 | 0.432437 |
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Target: 5'- -cGCCUGCUguaUGAUggGCACGCggCUa -3' miRNA: 3'- uaCGGAUGGag-ACUGuuUGUGCGa-GG- -5' |
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| 2528 | 5' | -52 | NC_001454.1 | + | 19468 | 0.72 | 0.392421 |
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Target: 5'- -cGaCUACCUCUGcgcuGCAAACAUGCUUUa -3' miRNA: 3'- uaCgGAUGGAGAC----UGUUUGUGCGAGG- -5' |
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| 2528 | 5' | -52 | NC_001454.1 | + | 32185 | 1.1 | 0.000933 |
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Target: 5'- aAUGCCUACCUCUGACAAACACGCUCCc -3' miRNA: 3'- -UACGGAUGGAGACUGUUUGUGCGAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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