miRNA display CGI


Results 1 - 4 of 4 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
2532 5' -53.4 NC_001460.1 + 22090 0.71 0.349325
Target:  5'- cUGCAAACCgcgCUUUUCUUCguugAGCCAUGUUg -3'
miRNA:   3'- -ACGUUUGG---GAGGAGAAG----UCGGUACGG- -5'
2532 5' -53.4 NC_001460.1 + 18335 0.71 0.367397
Target:  5'- aGCAAACCaCUgCaaugCAGCCGUGCUa -3'
miRNA:   3'- aCGUUUGG-GAgGagaaGUCGGUACGG- -5'
2532 5' -53.4 NC_001460.1 + 25782 0.69 0.488395
Target:  5'- cGUAaucaacuuAACCCUcCCUCUU-GGCCAgcUGCCc -3'
miRNA:   3'- aCGU--------UUGGGA-GGAGAAgUCGGU--ACGG- -5'
2532 5' -53.4 NC_001460.1 + 1971 1.12 0.000452
Target:  5'- aUGCAAACCCUCCUCUUCAGCCAUGCCc -3'
miRNA:   3'- -ACGUUUGGGAGGAGAAGUCGGUACGG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.