Results 1 - 9 of 9 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2535 | 3' | -49.4 | NC_001460.1 | + | 14851 | 0.66 | 0.892084 |
Target: 5'- aCGUAgGgACAGGuccGGCGGCGGGcgucaguGAUGGu -3' miRNA: 3'- -GUAUaCgUGUUC---UCGCCGCUC-------UUACC- -5' |
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2535 | 3' | -49.4 | NC_001460.1 | + | 21947 | 0.66 | 0.868251 |
Target: 5'- --gGUGCGCaAAGAGCauuuGGUGGaAAUGGa -3' miRNA: 3'- guaUACGUG-UUCUCG----CCGCUcUUACC- -5' |
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2535 | 3' | -49.4 | NC_001460.1 | + | 19649 | 0.67 | 0.821631 |
Target: 5'- aAUggGCACGcuGGUGGCGuuagcuGGGAUGGg -3' miRNA: 3'- gUAuaCGUGUucUCGCCGC------UCUUACC- -5' |
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2535 | 3' | -49.4 | NC_001460.1 | + | 17800 | 0.68 | 0.790769 |
Target: 5'- gAUGUGCAUguAAGAccacuGCGGCaucaucgaGGGAGUGGc -3' miRNA: 3'- gUAUACGUG--UUCU-----CGCCG--------CUCUUACC- -5' |
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2535 | 3' | -49.4 | NC_001460.1 | + | 15254 | 0.69 | 0.746979 |
Target: 5'- ----gGCGCAuuGGGCGGCGAGcuAUGa -3' miRNA: 3'- guauaCGUGUu-CUCGCCGCUCu-UACc -5' |
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2535 | 3' | -49.4 | NC_001460.1 | + | 22759 | 0.69 | 0.735654 |
Target: 5'- --aAUG-GCGGGAGCGGUGGGGGcGGu -3' miRNA: 3'- guaUACgUGUUCUCGCCGCUCUUaCC- -5' |
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2535 | 3' | -49.4 | NC_001460.1 | + | 2466 | 0.7 | 0.665651 |
Target: 5'- gGUAUGgGCccAGGGGUGGUGGGuuUGGa -3' miRNA: 3'- gUAUACgUG--UUCUCGCCGCUCuuACC- -5' |
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2535 | 3' | -49.4 | NC_001460.1 | + | 8053 | 0.71 | 0.606161 |
Target: 5'- ----aGCACGAGAGgGGCG-GAAacgGGa -3' miRNA: 3'- guauaCGUGUUCUCgCCGCuCUUa--CC- -5' |
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2535 | 3' | -49.4 | NC_001460.1 | + | 13093 | 1.13 | 0.001181 |
Target: 5'- aCAUAUGCACAAGAGCGGCGAGAAUGGg -3' miRNA: 3'- -GUAUACGUGUUCUCGCCGCUCUUACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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